权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Contamination and control of Pseudomonas aeruginosa between human being and laboratory animals

人与实验动物之间铜绿假单胞菌的污染及控制

基本信息

批准号：
03680041
负责人：
URANO Toru
金额：
$ 1.15万
依托单位：
KUMAMOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03680041/
关键词：
Pseudomonas aeruginosa laboratory animals mouse rat zoonosis epidemiology serological typing 疫学調査

项目摘要

Pseudomonas aeruginosa frequently contaminates specific pathogen-free laboratory animals. When the infected animals are given immunosuppressive agent or receive experimental stress, pseudomoniasis may be provoked. In addition, P. aeruginosa infection as well as pseudomoniasis is also a problem in hospital. The researchers who engage in animal experiment contact both patients and animals.So, we investigated the spread of P. aeruginosa between human being and laboratory animals.Examination sites : (1) Fingers and mouth of researchers and animal raisers before and after they enter the breeding room ; (2) The fresh faces and drinking water of animals ; (3) Washbasin and other 7 sites in the breeding room ; (4) Seven sites surrounding the breeding room.Examination methods : Isolation of P. aeruginosa by using NAC medium ; biochemical test for identification and then, serological typing by using antisera.Results : Many kinds of serotype of P. aeruginosa were isolated from the mouth of researchers. But, no P. aeruginosa was isolated from the animals used by the researchers and from the sites contacted with researchers. It is indicated that the researcher is not the infective source of P. aeruginosa for animals. Moreover, P. aeruginosa were isolated from the fingers of the researchers and animal raisers after they had finished experiment or breeding work. The serotype of the isolates is the same as that of isolates from the animals touched by them and that from the sites contaminated by them. This result shows that the researcher and animal raiser were contaminated of P. aeruginosa by animals and them became vehicle of infection.

铜绿假单胞菌经常污染无特定病原体的实验动物。当被感染的动物被给予免疫抑制剂或接受实验应激时，可能引起假单胞菌病。此外，铜绿假单胞菌感染以及假单胞菌病也是医院的一个问题。由于从事动物实验的研究人员既接触病人又接触动物，因此，我们调查了铜绿假单胞菌在人与实验动物之间的传播情况，检查部位：（1）研究人员和动物饲养员进入饲养室前后的手指和口腔;（2）动物新鲜的面部和饮水;（3）饲养室内的洗脸盆等7个部位;（4）饲养室周围7个地点，检测方法：采用NAC培养基分离铜绿假单胞菌，生化试验鉴定，再用抗血清进行血清学分型。但是，从研究人员使用的动物和与研究人员接触的地点中没有分离出铜绿假单胞菌。提示研究者不是动物铜绿假单胞菌的传染源。此外，铜绿假单胞菌是在研究人员和动物饲养者完成实验或养殖工作后从手指上分离出来的。分离株的血清型与接触动物分离株和污染场所分离株的血清型相同。结果表明，研究者和动物饲养者受到了动物的铜绿假单胞菌污染，成为感染的媒介。