MOLECULARBIOLOGY AND PHYSIOLOGY OF TASTE RECEPTION AND TRANSDUCTON USING DROSOPHILA TASTE GENE TRE.

建立了用爪蟾卵母细胞注射从肉蝇标签味觉器官中提取的多a或总RNA的体内表达体系。当用糖刺激物刺激果蝇的味觉感受器时，观察到一种向内的电流。在大多数情况下，对蔗糖或葡萄糖等糖的反应要高得多，而对甘露糖或乳糖等非刺激性糖的反应则较小或没有反应。从YAC克隆中分离出可能覆盖果蝇味觉基因Tre的基因组片段。由于该片段长度超过100 Kb，我们目前正在亚克隆更小的片段以鉴定该基因。通过记录标签感觉神经元，并与先前发表的行为结果进行比较，从生理学上分析了Tre的作用。

In vivo expression system using Xenopus oocyte by injecting polyA or total RNA extracted from labellar taste organs of the fleshfly has been established. An inward current was observed when the egg was perf used with sugar stimulants that stimulate fly taste receptors. In most cases the response was much higher for sugars like sucrose or glucose and smaller or absent for non-stimulating sugars like mannose or lactose.Genomic fragments that possibly cover the Drosophila taste gene Tre were isolated from YAC clones. Since the fragment contains more than 100 Kb length we are currently subcloning smaller fragments to identify the gene.The effect of Tre were analyzed physiologically by recording the labellar sensory neurons and compared with behavioral results that had been published earlier.