Development of a bead-ELISA kit for rapid identification of enteric bacteria

为了诊断细菌感染，有必要尽早识别引起细菌感染的细菌。然而，传统的细菌培养鉴定方法在获得临床资料后最短需要20-30小时。为了评价最近开发的一种高灵敏度的BEAD-EL ISA用于快速鉴定肠道感染的病原菌的工具，我们与印度霍乱和肠道疾病研究所合作，将BEAD-EL ISA应用于霍乱患者的粪便标本。结果表明，检测霍乱毒素的微球酶联免疫吸附试验是一种快速、敏感的检测霍乱毒素的方法，可用于霍乱患者的诊断。结果表明，该方法可用于空肠弯曲菌的鉴定。因此，将尝试开发一种空肠弯曲菌鉴定试剂盒。

To diagnose bacterial infection, it is necessary to identify the causative bacteria as early as possible. However, conventional methods to identify bacteria by using culture methods takes at shortest 20-30 hours after obtaining clinical materials.To evaluate a recently developed highly sensitive bead-ELISA to be used for a tool for the rapid identification of the causative bacteria of enteric infection, we cooperate with Institute of Cholera and Enteric Diseases of India and applied the bead-ELISA for stool specimens of cholera patients. The results showed that the bead-ELISA for cholera toxin is a rapid and sensitive method to detect cholera toxin, thus to diagnose cholera patient.Bead-ELISA system was also developed by using a specific antigen and whole cell antigen to compylobacter jejuni. It was demonstrated that the a developed bead-ELISA is useful to identify Campylobacter jejuni. Thus an attempt will be made to develop a kit for identification of Campylobacter jejuni.