Reconstitution of in vitro testing for metal allergy by means of LPT

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In order to reconstitution of in vitro testing (LPT:Lymphocyte Prolifiration Test) for metal allergy, following three series of investigation were made.1. Lymphocyte Prolifiration test: 1) Twelve highly positive reactors to patch test with Hg^<2+>, 5 highly positive reactors to patch test with Co^<2+> and 17 negative reactors to those, were participated in Lymphocyte Proliferation test(LPT). 2) A lymphocyte proliferation by Hg^<2+> was markedly depressed with 2-mercaptoethanol (2-ME) in the culture medium. 3) It was suggested that optimal incubation of metal sensitized lymphocyte was 144hs with Omol for Hg^<2+> and 120hs with 1.3mu-1.3muM for Co^<2+>.4) It was concluded that LPT might be applicable for sensitivity test to both Hg^<2+> and Co^<2+>, although further efforts should certainly be need to get results with gratified reliability in clinical purpose, especially in the former.2. Inducement of metal sensitized mice: Hg^<2+> sensitized mice(Balb/c) were successfully induced by subcutaneous or intraperitoneal injection with allergen-conbinant-epidermal cells.3. allergen-bind-fractions of the epidermal cell: After 24hs of application of 5% Hgcl^2 on skin surface of the mouse tail, amounts of Hg^<2+> bound with the microsome, with the mitochondria and with the lysosome of the epidermal cells were regarded as 40%, 33% and 23%, respectively.