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Assessment of Organic Acids in Whole Saliva as a risk factor of Dental Metal Allergy

全唾液中有机酸作为牙科金属过敏危险因素的评估

基本信息

批准号：
11470419
负责人：
FUJII Hiroyuki
金额：
$ 9.15万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470419/
关键词：
organic acid whole saliva metal prosthesis corrosion metal allergy cosmetic products household products risk factor 安静時唾液刺激時唾液唾液の臨床的特性無刺激全唾液

项目摘要

Metal ions released from metal of dental prosthesis and cosmetic and household products induce metal allergy. We suppose that the formation of soluble metal ions(correosion) in the mouth is affected by salivary characteristic. The first aims of this study was to determine concentration of organic acid in whole saliva , and* the second was to analyze containing metal element in cosmetic and household products from clinical viewpoint in metal allergy.Using EDXRF, containing metal elements in 81 different cosmetic and in 50 household products were analyzed. Resting and stimulated whole saliva were collected from 29 metal allergy patient and 44 dental patients who were free from any symptoms of metal allergy llfcitrol). Stimulated saliva induced by the chewing of paraffin pellet was collected by absorption onto cotton rolls for 5. Resting saliva samples were centrifuged at 3000 rpm for 15 min, and the resulting supernatants were frozen at -20℃ until used. Stimulate saliva samples were also frozen at -20℃. Salivary organic acids.that were phosphoric acid, citric acid, pyruvic acid, malic acid, succinic acid, lactic acid, formic acid, acetic acid, propionic acid and carbonic acid, were quantitatively determined by high-performance liquid chromatography method. Concentration of organic acid in whole saliva are statistically analyzed using Mann-Whitney U test.1. In mostofcosmetic and household products, Al, Si, Ti, Cr, Mn, Fe, Ni, Cu, Zn, Rb, Sr, Zr, Ba, Bi, and so on were clearly contained.2. For resting saliva metal allergy patient group had statistically significant higher concentration of pyruvic acid and carbonic acid than control group. While for stimulated saliva metal allergy patient group had statistically significant lower concentration of citric acid, acetic acid and propionic acid than control group.

假牙、化妆品和家居用品中金属释放的金属离子可引起金属过敏。我们认为，唾液的特性影响了口腔中可溶性金属离子（腐蚀）的形成。本研究首先测定了全唾液中有机酸的含量，其次从金属过敏的临床角度分析了化妆品和日用品中金属元素的含量，采用能量色散X射线荧光法对81种化妆品和50种日用品中的金属元素含量进行了分析。从29名金属过敏患者和44名没有任何金属过敏症状的牙科患者收集静息和刺激的全唾液。通过在棉卷上吸收收集由咀嚼石蜡颗粒诱导的刺激唾液5。将静息唾液样本以3000 rpm离心15 min，所得上清液在-20℃下冷冻备用。刺激唾液样本也在-20℃下冷冻。采用高效液相色谱法对唾液中的磷酸、柠檬酸、柠檬酸、苹果酸、琥珀酸、乳酸、甲酸、乙酸、丙酸和碳酸进行了定量分析。使用Mann-Whitney U检验对全唾液中的有机酸浓度进行统计学分析。在大多数化妆品和日用品中，Al、Si、Ti、Cr、Mn、Fe、Ni、Cu、Zn、Rb、Sr、Zr、Ba、Bi等元素含量明显.对于静息唾液，金属过敏患者组的唾液酸和碳酸浓度显著高于对照组。而刺激性唾液金属过敏患者组柠檬酸、乙酸和丙酸浓度显著低于对照组。