Development of a system for rapid protein structure

快速蛋白质结构系统的开发

• 批准号: 03558017
• 负责人: MITSUI Yukio
• 金额: $ 5.95万
• 依托单位: Nagoya University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03558017/
• 关键词: Imaging plate; X-ray structure analysis; Protein crystallography; Three- dimensional structure of protein; イメ-ジング・プレ-ト; X線回折計

1) system for rapid protein structure elucidation has been development making use of the two kinds of two-dimensional X-ray diffractometer employing "imaging plates" a) Laboratory-type diffractometer based on a commercial product, RIGAKU R-AXIS IIc and b) Weissenberg camera developed by Prof. Sakabe) installed at the synchrotron radiation source, Tsukuba.2) A system of the interface between a) X-ray diffraction data collected by the above two diffractometer systems and b) the computer graphic system already established in the present laboratory, has been established making use of the Ethernet data transfer linkage.3) Making use of the systems 1) and 2) described above, crystal structures for the following proteins have been either established (in a de novo fashion) or refined at a high resolution : Interferon-beta, RNase Rh, SSI-trypsin complex, Mutant SSI-subtilisin complex and Wild SSI-subtilisin complex.

1)利用两种二维X射线衍射仪开发了用于蛋白质结构快速鉴定的系统，所述两种二维X射线衍射仪使用了“成像板”a)基于商业产品的实验室型衍射仪，Rigaku R轴IIc和b)由Sakabe教授开发的Weissenberg相机)安装在同步辐射光源筑波。2)a)由上述两种衍射仪系统收集的X射线衍射数据与b)已经建立在本实验室中的计算机图形系统之间的接口系统已经利用以太网数据传输链路被建立。3)利用上述系统1)和2)利用上述系统1)和2下列蛋白质的晶体结构已经建立(以从头开始的方式)或以高分辨率精炼：干扰素-β、核糖核酸酶Rh、SSI-胰酶复合体、突变型SSI-枯草杆菌蛋白复合体和野生SSI-枯草杆菌菌素复合体。

项目成果

Y. Takeuchi, K. Miura & Y. Mitsui: "Molecular recognition at the active site of subtilisin BPN'" Protein Engineering. 4. 501-508 (1991)

Y.竹内、K.三浦

T. Senda, S. Matsuda & Y. Mitsui: "Three-dimensional crystal structure of recombinant murine interferon-beta" EMBO Journal. 11. 3193-32-1 (1992)

T.仙田、S.松田

T.NONAKA,Y.MITSUI,M.IRIE,K.T.NAKAMURA: "Three-dimensional structure of ribonuclease Ms^*3'-guanylic acid complex" FEBS Letters. 283. 207-209 (1991)

T.NONAKA、Y.MITSUI、M.IRIE、K.T.NAKAMURA：“核糖核酸酶 Ms^*3-鸟苷酸复合物的三维结构”FEBS Letters。

H. Kurihara, Y. Mitsui & K. T. Nakamura: "Crystal and molecular structure of RNase Rh, a new class of microbial ribonuclease" FEBS Letters. 306. 189-192 (1992)

H.栗原、Y.三井

Y.TAKEUCHI,K.MIURA.Y.MITSUI: "Molecular recognition at the active site of subtilisin BPN'" Protein Engineering. 4. 501-508 (1991)

Y.TAKEUCHI、K.MIURA.Y.MITSUI：“枯草杆菌蛋白酶 BPN 活性位点的分子识别”蛋白质工程。