HPLC Separation of Metal Chelates Utilizing Adduct Formation

利用加合物形成对金属螯合物进行 HPLC 分离

• 批准号: 03640486
• 负责人: TSUNODA Kin-ichi
• 金额: $ 1.28万
• 依托单位: Faculty of Engineering, Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03640486/
• 关键词: Adduct; HPLC; Metals; 4-(2-thienyl)-4-thioxo-1,1,1-trifluoro-2-butanone; 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione; 金属キレ-ト

The separation behavior of Co^<III>-, Ni^<II>-, Cu^<II>-, and Zn^<II>-STTAs [4-(2-thieny1)-4-thioxo-1,1,1-trifluoro-2-butanone] and Co^<II>-, Ni^<II>-, and Cu^<II>-TTAs [4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione] in reversed phase high performance liquid chromatography was investigated when various additives were added to the mobile phase. The adduct formation or the masking effect was observed upon addition of additives. When trioctylphosphine oxide was added to the mobile phase, mutual separation of those metal chelates was especially remarkable due to the adduct formation. Moreover, the decomposition of some metal chelates in the HPLC column was also prevented by the adduct formation. On the other hand, 2,2'-bipyridyl and 1,10-phenanthroline showed the masking effect. Such effects of additives were consistent with their synergistic extraction constants in chelate extraction.

研究了在流动相中加入不同添加剂时，Co^<III>-、Ni^<II>-、Cu^<II>-和Zn^<II>- sttas [4-(2-thieny1)-4-硫氧基-1,1,1-三氟-2-丁酮]和Co^<II>-、Ni^<II>-和Cu^<II>- ttas[4,4,4-三氟-1-（2-噻吩基）-1,3-丁二酮]在反相高效液相色谱中的分离行为。在添加添加剂后，观察到加合物的形成或掩蔽效应。当流动相中加入氧化三辛基膦时，由于加合物的形成，这些金属螯合物的相互分离尤为显著。此外，加合物的形成也阻止了某些金属螯合物在高效液相色谱柱中的分解。而2,2′-联吡啶和1,10-菲罗啉则表现出掩蔽作用。添加剂的这种作用与其在螯合物萃取中的协同萃取常数一致。