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A STUDY OF IDENTIFICATION AND QUANTIFICATION OF MIDDLE REPETITIVE SEQUENCES OF DROSOPHILA GENOME

果蝇基因组中间重复序列的鉴定和定量研究

基本信息

批准号：
03640540
负责人：
HARADA Ko
金额：
$ 1.22万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03640540/
关键词：
middle repetitive sequence Drosophila melanogaster transposable elements random clone in situ hybridilzation genome structure in situ hybridization ランダムクロ-ンオナジショウジョウバエ

项目摘要

In order to study the genome structure concerning to the repetitive sequences we mapped random clones extracted from the genomic library of Drosophila melanogaster on the salivary gland chromosome. About 800 clones were randomly chosen from the phage library and chromosomal locations were determined for 231 clones by in situ hybridization. 142 clones were mapped on the unique locations and 89 clones were mapped on the multiple chromosomeal locations. Two groups of clones were distinguished from the distribution of these clones containing sequences which have multiple copies on the chromosome. One group is made of clones which has relatively small copy numbers (2-6) and the others have more than 10 copies on the chromosome. Mapping these clones to the chromosome of Drosopila simulans, the closely related species of D.melanogaster, it was found that the former group of clones were mapped on the same location and/or have the same copy numbers. On the other hand, the latter had generally smaller copy numbers and were mapped on different locations. These results suggest that the former clones are made of mainly multigene families and the latters contains transposons. We are now analyzing detailed molecular structure of these clones.

为了研究与重复序列相关的基因组结构，我们将从黑腹果蝇基因组文库中提取的随机克隆定位在唾液腺染色体上。从噬菌体文库中随机挑选了约800个克隆，通过原位杂交确定了231个克隆的染色体位置。142个克隆被定位在唯一位置，89个克隆被定位在多个染色体位置。从这些克隆的分布情况可以区分出两组克隆，这些克隆包含在染色体上具有多个拷贝的序列。一组由拷贝数相对较少(2-6个)的克隆组成，另一组在染色体上有10多个拷贝。将这些克隆定位于Drosopila Simans的染色体上，发现前一组克隆被定位在相同的位置和/或具有相同的拷贝数。另一方面，后者的拷贝数通常较小，并被映射到不同的位置。这些结果表明，前者主要由多基因家族组成，后者含有转座子。我们现在正在分析这些克隆的详细分子结构。