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Molecular population genetic study of the regulatory systems of the heat shock genes

热休克基因调控系统的分子群体遗传学研究

基本信息

批准号：
06640799
负责人：
HARADA Ko
金额：
$ 1.41万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06640799/
关键词：
Heat shock gene HSP HSF Molecular evolution Drosophila melanogaster multigene Enrancertrag Transcription regulation

项目摘要

The regulatory system of the heat shock genes are not only an attractive model for the study of the regulatory mechanism of transcription in higher organisms, but also an excellent material for studying the adaptive evolution of transcription regulation system. Many molecular knowledge have been accumulated on the regulatory mechanism of transcription of heat shock genes in Drosophila, yeast and humans.Based on these knowledge, we planed to study the adaptive evolution of the regulatory system in the heat shock genes. We planed the following experiments using Drosophila melanogaster as materials : (1) Survey of unknown factors which regulate the transcription of heat shock genes, (2) molecular cloning of the transcription factor, HSF and (3) molecular population genetics of genetic variation within and between the Drosophila species. We also studied the theoretical aspects of molecular evolution of maltigene families including heat shock genes. We obtained the following results : (1) We established about 4000 enhancer trap strains using P transposon. We surveyed mutant strains under the heat and cold shock treatment. We obtained about 10 such straing and continuing searching. (2) We cloned four HSF gene clones from Drosophila genomic library. We are now analyzing the molecular structure of these clones. (3) We sequenced PCR products of HSF for several populations of Drosophila melanogaster and other sibling species. We obtained some of the results but not completed. For the theoretical study, we did computer simulation and studied the polymorphic level of multigenes at various population sizes including the effects of mutations, unequal crossing overs, genetic drift and natural selection.

热休克基因的调控系统不仅是研究高等生物转录调控机制的一个有吸引力的模型，也是研究转录调控系统适应性进化的极好材料。关于果蝇、酵母和人类热休克基因转录调控机制已经积累了很多分子知识。基于这些知识，我们计划研究热休克基因调控系统的适应性进化。我们以果蝇为材料计划了以下实验：（1）调节热休克基因转录的未知因子的调查，（2）转录因子、HSF的分子克隆和（3）果蝇种内和种间遗传变异的分子群体遗传学。我们还研究了包括热休克基因在内的麦芽基因家族分子进化的理论方面。我们得到了以下结果：(1)利用P转座子建立了约4000个增强子捕获菌株。我们调查了热休克和冷休克处理下的突变株。我们获得了大约 10 个这样的紧张和持续搜索。 (2)我们从果蝇基因组文库中克隆了4个HSF基因克隆。我们现在正在分析这些克隆的分子结构。 (3) 我们对果蝇和其他同胞物种的几个种群的 HSF PCR 产物进行了测序。我们获得了一些结果，但尚未完成。对于理论研究，我们进行了计算机模拟并研究了不同群体规模下多基因的多态性水平，包括突变、不平等交换、遗传漂变和自然选择的影响。