Regulation of photosynthesis

光合作用的调节

• 批准号: 03640576
• 负责人: OKADA Mitsumasa
• 金额: $ 1.41万
• 依托单位: Faculty of Soience, Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03640576/
• 关键词: Bryopsis maxima; Intron; Photosynthesis; rbcL; RuBisCO; ダナリエラ; ピレノイド; DNA; スタ-チシンタ-ゼ; デンプン; 光制御; 緑藻オオハネモ; ルビスコ遺伝子

1) Pyrenoids of Bryopsis maxima contained several minor components other than the large subunit (LS) and the small subunit of ribulose 1, 5-bisphosphate carboxylase /oxygenase (RuBisCO). Among the minor components, polypeptides of 95, 67, and 41 kDa reacted with and antiboby against the LS polypeptide. Amino acid sequences of these polypeptides were determined and compared with that deduced from the LS gene (rbcL) screened from the chloroplasts DNA library of B.maxima. The N-terminal sequence of the LS peptide was not post-translationally processed and was almost identical with those of the polypeptides of 91, 67, and 41 kDa. The starch grains surroundeing the pyrenoisd contained a polypeptide of 66 kDa that was assigned as starch synthase.2) rbcL of B.maxima contained 1425 bp coding 475 amino acids. An intron of 2467 bp was found between codon 268 and 269.3) Transcription of rbcL to m-RNA was enhanced in light condition, suggesting the regulation of photosynthetic activity by light. Northen hybridization using two exons and one intron as probes showed the persence of m-RNAs with the size of 0.7 and 1.6 Kpb respectively.The intron and the whole rbcL were ligated with vectors and inserted into the host E.Coli. The expression of peptides was observed.

1)除了核酮糖1，5-二磷酸羧基/加氧酶的大亚基(LS)和小亚基(Rubisco)外，Bryopsis Maxima的果核类化合物还含有其他几种次要成分。在次要成分中，95、67和41 kDa的多肽与LS多肽反应，并与LS多肽产生抗体。测定了这些多肽的氨基酸序列，并与从叶绿体DNA文库中筛选出的LS基因(RbcL)的氨基酸序列进行了比较。LS多肽的N-末端序列没有翻译后加工，与91、67和41 kDa的多肽几乎完全相同。蛋白核周围的淀粉粒含有一个66 kDa的多肽，被认为是淀粉合成酶。2)极大拟青霉rbc1含有1425个核苷酸，编码475个氨基酸。在268-269.3密码子之间有一个2467bp的内含子)，在光照条件下，rbcL转录为m-RNA增强，表明光对光合作用活性有调节作用。以两个外显子和一个内含子为探针的Norten杂交显示，m-RNAs的大小分别为0.7kPB和1.6kPB，内含子和整个rbcL分别与载体连接并插入到宿主大肠杆菌中。观察多肽的表达情况。

项目成果

H.Ohsawa,K.Nakayama,and M.Okada: "Adenylate cyclase and phosphodies ferase activities in reiation to a rapid in crease in cellular CAMP cmcentration by hyper tcnic shock in D.rivid.s" Bot.Mag.Tokyo. 105. 393-404 (1992)

H.Ohsawa、K.Nakayama 和 M.Okada：“腺苷酸环化酶和磷酸二酯酶活性与 D.rivid.s 中超强休克引起的细胞 CAMP 浓度快速增加有关”Bot.Mag.Tokyo。

M.Okada, Y.Okabe, M.kono, K.Nakayama and H.Satoh: "Peptide composition and enzyme activities of isolated pyrenoids from the green alga Bryopsis maxima" Can.J.Bot.69. 1053-1061 (1991)

M.Okada、Y.Okabe、M.kono、K.Nakayama 和 H.Satoh：“从绿藻 Bryopsis maxima 中分离出的蛋白核的肽组成和酶活性”Can.J.Bot.69。

M.Okada: "Progress in Phycological Research 8" 117-138 (1992)

M.Okada：《藻类研究进展8》117-138（1992）

H. Ohsawa,K.Nakayama and M.Okada: "Adenyla te cyclase and phosphodiesterase activities in relation to a rapid increase in cellular CAMP concentration by hyperlonic Shock in D. viridis" Bot. Mag. Tokyo. 105. 393-404 (1992)

H. Ohsawa、K.Nakayama 和 M.Okada：“腺苷酸环化酶和磷酸二酯酶活性与 D. viridis 中高渗休克引起的细胞 CAMP 浓度快速增加有关”Bot。

M.Kono,H.Satoh,Y.Okabe,Y.Abe,K.Nakayama and M.Okada: "Nucleotide sequence of the large subunit of riduloseー1,5ーbisphosーphate carboxylase/oxygenase from the green alga Bryopsis maxima." Plant Molecular Biology. 17. 505-508 (1991)

M.Kono、H.Satoh、Y.Okabe、Y.Abe、K.Nakayama 和 M.Okada：“来自绿藻 Bryopsis maxima 的 Ridulose-1,5-bisphos-phate 羧化酶/加氧酶大亚基的核苷酸序列”植物分子生物学。17。505-508（1991）