权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Purification and Physiological role of the adenylate cyclase in Dunaliella

杜氏藻腺苷酸环化酶的纯化及其生理作用

基本信息

批准号：
05640737
负责人：
OKADA Mitsumasa
金额：
$ 1.28万
依托单位：
Toho University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05640737/
关键词：
Adenylate cyclase Dunaliella Salt tolerance

项目摘要

1) Growth and Photosynthetic activity in Dunaliella under stress.Signal transduction system including adenylate cyclase (AC) is supposed to be responsible for some metabolic changes in Dunaliella. The alga showed following changes according to salt stress, aging and high light.・In the medium containing 10% NaCl, the maximum growth was observed in ten days. In that containing 25% NaCl, slow growth was observed during 30 days. Maximum cell numbers per ml of medium was about 20 times larger in the former medium.・During aging, decrease of chlorophyll content and accumulation of carotenoids, especiaslly beta-carotenoids were obseved.・Chlorophyll a/b ratio increased from 1.8 to 3.5 when light-intensity for the culture increased. The change of the efficincy for the energy transfer from carotenoids and chl b to chl a was also observed.2) AC in Dunaliella showed remarkable affected by divalent cations. No activity was observed in the absence of Mg and the activity was fully enhanced at 5mM of Mg. Ca showed strong inhibition and 50% inhibition occurred at 50-100uM.3) Membrane fraction was treated by 1M NaCl, and solubilized AC was purified by gel filtration followed by ion change chromatography. Molecular wight of whole enzyme was 452kDa and that of the subunit was estimated 116kDa by SDS-PAGE.

1)盐藻在逆境条件下的生长和光合活性研究表明，盐藻的某些代谢变化与腺苷酸环化酶（AC）等信号转导系统有关。在盐胁迫、老化和强光胁迫下，黄瓜表现出以下变化。在含10%NaCl的培养基中，10天后生长量最大。在含25%NaCl的培养液中，30天内生长缓慢。在前一种培养基中，每ml培养基的最大细胞数约为20倍。·在老化过程中，叶绿素含量下降，类胡萝卜素，特别是β-类胡萝卜素积累减少。叶绿素a/B比值随光照强度的增加而增加，由1.8增加到3.5。2）盐藻AC受二价阳离子的影响较大，但对类胡萝卜素和叶绿素B向叶绿素a的能量传递效率影响不大。在不存在Mg的情况下没有观察到活性，并且在5 mM的Mg下活性完全增强。Ca对活性炭有较强的抑制作用，在50- 100 μ M浓度范围内抑制率可达50%。3）用1 M NaCl溶液处理膜组分，经凝胶过滤和离子交换层析分离纯化。SDS-PAGE测得酶的分子量为452 kDa，亚基分子量为116 kDa。