Hybridization of Functional Bioabsorbable Polymers with Bioactive Compounds and Biocells

功能性生物可吸收聚合物与生物活性化合物和生物细胞的杂交

• 批准号: 03650750
• 负责人: KIMURA Yoshiharu
• 金额: $ 1.28万
• 依托单位: Kyoto Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03650750/
• 关键词: Bioabsorbable polymer; Malic acid; RGD tripeptide; Polyester-peptide conjugate; Cell culture; L-lactide; Cell attachment; ポリエステル-ペプチド複合体; RGD; ポリエステルーペプチド複合体; ゼラチン

A new poly(alpha-hydroxy acid) comprising alpha-malic acid was synthesized by copolymerization of 3-(s)-[(benzyloxycarbonyl)methyl]-1,4-dioxane-2,5-dione (BMD) and L-lactide followed by deprotection of the benzyl groups by hydrogenolysis. The resultant copoly(alpha-hydroxy acid)(PMLA), consisting of glycolic acid (G), alpha-malic acid (M), and L-lactic acid (L) units, showed an accelerated hydrolysis rate due to the action of the pendant carboxyl groups. These carboxyl groups were also utilized as the sites of chemical modification to obtain a bioresorbable polymer with intended functions. Here, RGD tripeptide was immobilized on these carboxyl groups by DCC method in order to enhance the cell attachment property. of PGML. The hybrid materials of this RGD-immobilized bioresorbable polymer and biocells have a high potential for developing guided tissue regeneration (GTR) technique in which regeneration of human tissue is induced and controlled on a specially designed biodegradable polymeric matrix. Also, the effect of polymer eroding on the cell attachment and growth was analyzed.

以3-（S）-[（苄氧羰基）甲基]-1，4-二氧六环-2，5-二酮（BMD）和L-丙交酯为原料，通过共聚反应，再通过氢解脱保护苄基，合成了一种新型的聚α-羟基酸，其结构为α-苹果酸。由乙醇酸（G）、α-苹果酸（M）和L-乳酸（L）单元组成的所得共聚（α-羟基酸）（PMLA）由于侧羧基的作用而显示出加速的水解速率。这些羧基也被用作化学修饰的位点，以获得具有预期功能的生物可吸收聚合物。在此，通过DCC法将RGD三肽固定在这些羧基上，以增强细胞粘附性。的PGML。这种RGD固定的生物可吸收聚合物和生物细胞的混合材料具有开发引导组织再生（GTR）技术的高潜力，其中在特别设计的生物可降解聚合物基质上诱导和控制人体组织的再生。分析了聚合物侵蚀对细胞贴壁生长的影响。

项目成果

Y. Kimura: "Degradation of azo-containing poyurethane by the action of intestinal flora: its mechanism and application as a drug delivery system" Polymer. 33-24. 5294-5299 (1992)

Y. Kimura：“肠道菌群作用下含偶氮聚氨酯的降解：其机制及其作为药物输送系统的应用”聚合物。

木村 良晴: "Copolymer:2ation of 3-(S)-[(benzyloxycarbonyl)methyl]-1.4-dione-2.5-dione and L-lactide:a facile synthetic method for functional:2ated bioabsorbable polymer" Polymer. 34. (1993)

Yoshiharu Kimura：“共聚物：3-(S)-[(苄氧基羰基)甲基]-1.4-二酮-2.5-二酮和 L-丙交酯的 2 化：功能性：2 化生物可吸收聚合物的简便合成方法”Polymer 34。 ）

木村 良晴: "Copolymerization of 3-(S)-[(bonzyloxgcarbongl)methyl]-1,4-dioxane-2,5-dione and L-lactide:a facile synthetic method for functionalized bioabsorbable polymer" Polymer. 34. (1993)

Yoshiharu Kimura：“3-(S)-[(bonzyloxgcarbongl)methyl]-1,4-dioxane-2,5-dione 和 L-丙交酯的共聚：功能化生物可吸收聚合物的简便合成方法”Polymer 34。 ）

木村 良晴: "Degradation of azoーcontaining polyurethane by the action of intestinal frora:Its mechanism and application for drug delivery ystem" Polymcr. 33. (1992)

Yoshiharu Kimura：“肠道菌群作用下含偶氮聚氨酯的降解：其机制及其在药物输送系统中的应用”Polymcr. 33。

林 寿郎・木村 良晴: "高分子機能材料シリ-ズ第9巻 医寮機能材料" 共立出版, 482 (1990)

Toshiro Hayashi和Yoshiharu Kimura：《高分子功能材料系列第9卷：医疗宿舍功能材料》Kyoritsu Shuppan，482（1990）