Control mechanisim of the electron transport in chloroplasts

叶绿体电子传递的控制机制

基本信息

批准号：
04044110
负责人：
MATSUBARA Hiroshi
金额：
$ 4.99万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Saeki et al. have constructed mutated fdxN of R. capsulatus with gene-engineering techniques. They identified cysteine residues essential to activity. With EPR spectroscopy, the spin interaction was found to be strongly affected by the amino acid residues around Fe-S clusters. He also demonstrated that the structures of fdxA product around [3Fe-4S] cluster and carboxyl terminal region were important to function physiologically.Oh-oka et al. have succeeded in purifying the Chlorobium reaction center complex (CRC-2) made of two subunits, that is, the core protein (68kDa) and cytochrome c551(21kDa), respectively. Laser flash photolysis revealed the primary charge separation, followed by the delayed fluorescence concomitant with the formation of P840^T. They also have cloned the cytochrome c551 gene, and intend to construct transformation system in Chlorobium.Wada have isolated two FNR isoenzymes from radish leaf and root. Antibodies against them were raised in rabbits. Each antibody showed specific reactivity and had no cross-reactivity. The antibodies distinguished the enzyme forms from roots and leaves of radish as well as other flowering plants. The FNR isoenzymes showed organ-specific distribution.Buchanan reported that the level of thioredoxin in the endosperm might be under hormonal control during germination. He also demonstrated that spinach thioredoxin m inhibits DNA synthesis in fertilized Xenopus eggs.Malkin have recently cloned the petC, which encoded Rieske protein. He intends to construct site-directed mutagenesis of the petC in order to study the cyclic electron transport with EPR technique.

Saeki等。已经用基因工程技术构建了R. capsulatus突变的FDXN。他们确定了活性必不可少的半胱氨酸残基。通过EPR光谱法，发现自旋相互作用受到Fe-S簇附近的氨基酸残基的强烈影响。他还证明了[3FE-4]簇和羧基末端区域周围FDXA产品的结构对于生理功能很重要。OH-OKA等人。分别成功地纯化了由两个亚基制成的叶绿素反应中心络合物（CRC-2），即分别是核心蛋白（68KDA）和细胞色素C551（21KDA）。激光闪光光解会显示主要电荷分离，然后延迟荧光与p840^t的形成。他们还克隆了细胞色素C551基因，并打算在叶绿体中构建转化系统。WADA已从萝卜叶和根中分离了两个FNR同工酶。针对它们的抗体是在兔子中提出的。每种抗体表现出特定的反应性，没有交叉反应性。抗体将酶形成与萝卜的根和叶子以及其他开花植物区分开。 FNR同工酶显示出器官特异性的分布。布坎南报道，胚乳中的硫氧还蛋白水平可能在发芽期间受到激素对照。他还证明了菠菜硫氧还蛋白M可抑制受精的爪蟾卵中的DNA合成。Malkin最近将PETC克隆，该PETC编码了Rieske蛋白。他打算构建PETC的位置定向诱变，以便使用EPR技术研究环状电子传输。