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Control mechanisim of the electron transport in chloroplasts

叶绿体电子传递的控制机制

基本信息

批准号：
04044110
负责人：
MATSUBARA Hiroshi
金额：
$ 4.99万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Saeki et al. have constructed mutated fdxN of R. capsulatus with gene-engineering techniques. They identified cysteine residues essential to activity. With EPR spectroscopy, the spin interaction was found to be strongly affected by the amino acid residues around Fe-S clusters. He also demonstrated that the structures of fdxA product around [3Fe-4S] cluster and carboxyl terminal region were important to function physiologically.Oh-oka et al. have succeeded in purifying the Chlorobium reaction center complex (CRC-2) made of two subunits, that is, the core protein (68kDa) and cytochrome c551(21kDa), respectively. Laser flash photolysis revealed the primary charge separation, followed by the delayed fluorescence concomitant with the formation of P840^T. They also have cloned the cytochrome c551 gene, and intend to construct transformation system in Chlorobium.Wada have isolated two FNR isoenzymes from radish leaf and root. Antibodies against them were raised in rabbits. Each antibody showed specific reactivity and had no cross-reactivity. The antibodies distinguished the enzyme forms from roots and leaves of radish as well as other flowering plants. The FNR isoenzymes showed organ-specific distribution.Buchanan reported that the level of thioredoxin in the endosperm might be under hormonal control during germination. He also demonstrated that spinach thioredoxin m inhibits DNA synthesis in fertilized Xenopus eggs.Malkin have recently cloned the petC, which encoded Rieske protein. He intends to construct site-directed mutagenesis of the petC in order to study the cyclic electron transport with EPR technique.

Saeki等人利用基因工程技术构建了荚膜菌的fdxN突变体。他们确定了对活性至关重要的半胱氨酸残基。通过EPR谱分析，发现Fe-S簇周围氨基酸残基对自旋相互作用有强烈的影响。他还证明了fdxA产物在[3Fe-4S]簇周围和羧基末端区域的结构对生理功能很重要。Oh-oka等人成功纯化了由核心蛋白（68kDa）和细胞色素c551（21kDa）两个亚基组成的氯酸反应中心复合物（CRC-2）。激光闪光光解反应显示了初级电荷分离，随后是伴随P840^T形成的延迟荧光。他们还克隆了细胞色素c551基因，并打算在绿藻中构建转化体系。Wada从萝卜叶和根中分离出两种FNR同工酶。在兔子体内培养了针对它们的抗体。各抗体均具有特异性反应性，无交叉反应性。抗体将这种酶从萝卜的根和叶以及其他开花植物中区分出来。FNR同工酶呈器官特异性分布。Buchanan报道胚乳中硫氧还蛋白的水平可能在萌发过程中受激素控制。他还证明了菠菜硫氧还蛋白m能抑制爪蟾受精卵的DNA合成。Malkin最近克隆了编码Rieske蛋白的petC。他打算构建petC的定点诱变，以便利用EPR技术研究其循环电子传递。