Studies on Multilateral Utilization of Microalgae

微藻的综合利用研究

• 批准号: 04304020
• 负责人: YAMAGUCHI Katsumi
• 金额: $ 11.52万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04304020/
• 关键词: Microalgae; Utilization; Fuel hydrocarbon; DNA-taxonomic marker; Organic sulfur compounds; Live feeds for aquaculture; Ultrafine calcite particles; Biologically active compounds; 餌料

Studies on multilateral utilization of microalgae have been carried out with the following results :(1)The search for new strains of the hydrocarbon-producing green microalga Botryococcus brauniiwas attempted in the fields to obtain one A-race strain and 4 B-race ones. Two of them exhibited a hydro carbon content above 30% of the dry weight.(2)The total DNA was extracted and purified from 30 strains of 7 species of Alexandrium. The 5.8SrDNA and flanking ITSs sequences were PCR-amplified from the purified total DNA.(3)Methionine was shown to be an efficient precursor for the biosynthesis of dimethylthiopropanoic acid(DMTP)in the dinoflagellate Crypthecodinium Cohnii.(4)The effects of coexistence of bacterial strains isolated from a Kuruma prawn farm on the growth of the diatom Chaetoceros gracilis were investigated. Flavobacterium sp.and Moraxlla sp.had the promotive effect markedly and slightly, respectively.(5)A new procedure for the production of ultrafine calcite particles by the marine coccolithophorid alga Pleuroc hrysis carterae was developted, in which the particles(coccoliths)were detached from the cell surface by optimized air-bubbling.(6)Aeruginosin 298-A,a potent thrombin and trypsin inhibitor, was isolated from the freshwater blue-green alga Microcystis aeruginosa(NIES-298). Its structure and activity were elucidated.(7)Gambieric acids, antifungal substances, were isolated from the marine dinoflagellate Gambierdiscus toxicus. Their potency exceeded that of amphotericin-B 2000-fold.

对微藻的综合利用进行了研究，结果如下：(1)对产烃绿色微藻巴西芽孢杆菌的新菌株进行了野外筛选，获得了1株A、4株B小种。提取和纯化了亚历山大藻7种30株的总DNA。从纯化的总DNA中扩增出5.8SrDNA及其两侧的ITSS序列。(3)蛋氨酸是甲硫氨酸生物合成二甲硫丙酸(DMTP)的有效前体。(4)研究了从库鲁马对虾养殖场分离的细菌对硅藻生长的影响。黄杆菌属和莫拉氏菌属分别具有显著和轻微的促进作用。(5)开发了一种利用海洋球藻金黄色侧耳(Pleuroc Hrysis Carterae)生产超细方解石的新工艺。(6)从淡水蓝藻铜绿微囊藻(NIES-298)中分离得到一种有效的凝血酶和胰酶抑制因子--铜绿球蛋白298-A。(7)从海洋甲藻Gbierdisus toxicus中分离得到抗真菌物质冈比亚酸。它们的效力超过两性霉素-B 2000倍。

项目成果

M.Adachi 他2名: "Restriction Fragment. Leugth Polymorphism of Ribosomol DNA Internal Trauscribed Spacer and 5.85 Regions in Japanese Alexandrium Specles(Dinophyceae)" J.Phycol.30. 857-863 (1994)

M.Adachi 和其他 2 人：“限制性片段。日本亚历山大藻（甲藻）中核糖体 DNA 内部转录间隔区和 5.85 区域的 Leugth 多态性”J.Phycol.30 (1994)。

Suminto 他1名: "Relation between Diatom Growth and Bacterial Population in Sewi Hass Culture Tanks of Diatom." Bull.Fac.Fish.,Nagasaki Univ.74/75. 37-42 (1993)

Suminto 等人：“Sewi Hass 硅藻培养箱中硅藻生长与细菌数量的关系”，Nag​​asaki Univ.74/75 (1993)。

A.Uchida: "Incorporation of Methionine into Dimethylthiopropanoic Acid in the Dinoflagellate Cryphecodinium cohnii" Nippon Suisan Gakkaishi. 59,5. 851-855 (1993)

A.Uchida：“甲硫氨酸与甲硫基丙酸在甲藻隐甲藻中的结合”Nippon Suisan Gakkaishi。

A.Uchida 他3名: "Iucorporation of Mcthionine into Dimethyl thiopropanoic Acid in the Dinof lagellato Cryphecodinium cohnii" Nippon Suisan Gakkaishi. 59. 851-855 (1993)

A. Uchida 和其他 3 人：“Dinof lagellato Cryphecodinium cohnii 中甲硫氨酸与二甲基硫代丙酸的结合”Nippon Suisan Gakkaishi 59. 851-855 (1993)。

Suminto: "Relation between Diatom Growth and Bacterial Population in Semi Mass Culture Tnaks of Diatom" Bull.Fac.Fish., Nagasaki Univ.74/75. 37-42 (1993)

Suminto：“硅藻半批量培养中硅藻生长与细菌种群的关系”Bull.Fac.Fish.，长崎大学74/75。