Studies on Body Color Expression of Cultured Black Tiger, Prawn

养殖黑虎、虾体色表现的研究

• 批准号: 06454101
• 负责人: YAMAGUCHI Katsumi
• 金额: $ 4.54万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454101/
• 关键词: Black Tiger Prawn; Carotenoid; Astaxanthin; Carotenoprotein

Carotenoids and carotenoproteins in the exoskeleton and the muscular epithleium of cultured black tiger prawn Penaeus monodon were analyzed to establish a strategy to improve their body color. First, carotenoid content and composition in the exoskeleton of the prawns exhibiting different body colors were determined. A consistency was observed between the depth of body color and the carotenoid content of 2.3-33.1mg/100g in the exoskeleton. The major carotenoid, astaxanthin, existed in three forms, namely diester, monoester, and free ones. Second, a blue carotenoprotein of 120kDa was purified from the exoskeleton. Another of 21.4kDa was also isolated from the muscular epithelium with a collagenase solution. Finally, carotenoids in both fractions were analyzed and compared between dark gray and blue prawns. A blue carotenoprotein fraction was liberated from the muscular epithelium by extraction with a collagenase solution, leaving another carotenoid fraction in the tissue. The main carotenoid in the carotenoprotein fraction was free astaxanthin, of which content showed no significant difference between the two prawn groups. The muscular epithelium after removal of blue carotenoprotein turned red owing to the remaining carotenoids which were composed largely of astaxanthin esters. The total content of the remaining red carotenoids was about six-fold higher in the dark gray than blue group. Thus the different body color of cultured black tiger prawns is suggested to be due to the varied composition of the blue carotenoprotein and red carotenoid fractions in the muscular epithelium.

对养殖斑节对虾的外骨骼和肌肉上皮中的类胡萝卜素和胡萝卜素蛋白进行了分析，以建立改善其体色的策略。首先，测定了表现出不同体色的虾的外骨骼中的类胡萝卜素含量和组成。体色深浅与外骨骼中类胡萝卜素含量2.3-33.1mg/100g之间观察到一致性。主要的类胡萝卜素虾青素以三种形式存在，即二酯、单酯和游离形式。其次，从外骨骼中纯化出 120kDa 的蓝色胡萝卜素蛋白。还用胶原酶溶液从肌肉上皮中分离出另一个 21.4kDa。最后，对深灰色和蓝色虾的两个部分中的类胡萝卜素进行了分析和比较。通过用胶原酶溶液提取，从肌肉上皮中释放出蓝色的胡萝卜素蛋白部分，在组织中留下另一种类胡萝卜素部分。胡萝卜素蛋白组分中的主要类胡萝卜素是游离虾青素，其含量在两组对虾之间没有显着差异。去除蓝色胡萝卜素蛋白后，肌肉上皮由于剩余的类胡萝卜素主要由虾青素酯组成而变成红色。深灰色组中剩余红色类胡萝卜素的总含量比蓝色组高约六倍。因此，养殖黑虎虾的不同体色被认为是由于肌肉上皮中蓝色胡萝卜素蛋白和红色类胡萝卜素部分的不同组成造成的。

项目成果

S.A.Nur-E-Borhan: "Carotenoprotein from the Exoskeleton and the Muscular Epithelium of the Black Tiger Prawn Penaevs monodon" Fisheries Science. 61(2). 337-343 (1995)

S.A.Nur-E-Borhan：“来自斑节对虾黑虎虾的外骨骼和肌肉上皮的胡萝卜素蛋白”渔业科学。

Shigeru Okada: "Changes in Body Color Appearance of the Black Tiger Prawn Penaeus monodon by the Varied Composition of Carotenoids Soluble as Carotenoprotein and Remaining Insoluble after Collagenase Treatment for the Nuscular Epithelium" Fisheries Scienc

Shigeru Okada：“不同类胡萝卜素成分对斑节对虾体色外观的变化，类胡萝卜素可作为胡萝卜素蛋白溶解，但在胶原酶处理核上皮细胞后仍保持不溶解状态”渔业科学

S.A.Nur-E-Borhan et al.: "Carotenoproteins from the Exoskeleton and the Muscular Epithelium of the Black Tiger Prawn Penaeus monodon" Fisheries Science. 61. 337-343 (1995)

S.A.Nur-E-Borhan 等人：“来自斑节对虾黑虎虾的外骨骼和肌肉上皮的胡萝卜素蛋白”渔业科学。

Shigeru Okada: "Carotenoid Composition in the Exoskeleton of Commercial Black Tiger Prawn" Fisheries Sciepce. 60(2). 213-215 (1994)

Shigeru Okada：“商业黑虎虾外骨骼中的类胡萝卜素成分”渔业科学。

Shigeru Okada et al.: "Changes in Body Color Appearance of the Black Tiger Prawn Penaeus monodon by the Varied Composition of Carotenoids Soluble as Carotenoprotein and Remaining Insoluble after Collagenase Treatment for the Muscular Epithelium" Fisheries

Shigeru Okada 等人：“不同类胡萝卜素组合物对斑节对虾体色的影响