Role of Limulus Hemocytes in the Biological Defense System.

鲎血细胞在生物防御系统中的作用。

基本信息

  • 批准号:
    04404090
  • 负责人:
  • 金额:
    $ 17.28万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (A)
  • 财政年份:
    1992
  • 资助国家:
    日本
  • 起止时间:
    1992 至 1994
  • 项目状态:
    已结题

项目摘要

Horseshoe Crab hemocyte lysate responds to (1*3) -beta-D-glucans, initiating an enzymatic cascade which culminates in clot formation. We have purified to homogeneity the serine protease zymogen, factor G,which is directly activated by (1*3) -D-glucans and which initiates the hemolymph clotting cascade. Factor G is a heterodimeric protein composed of two noncovalently-asociated subnits alpha (72 kDa) and beta (37 kDa). In the prescence of (1*3) -D-glucans such as curdlan and paramylon, factor G is autocatalytically activated to an active serine protease, named factor G.This activation is accompanied by limited proteolyses of Arg-X bonds in both subunits : the 72-kDa subunit alpha is cleaved to 55-kDa and 17-kDa fragments, and the 37-kDa subunit beta is shortened to 34-kDa. Reconstitution experiments using purified proteins participating in the hemolymph clotting cascade demonstrate that factor G is capable of activating proclotting enzyme directly, resulting in the conversion of coagulo … More gen to coagulin gel. Analyzes of cDNAs for both subunits indicates that the subunits are derived from separate mRNA species and thus encoded by different genes. Subunit beta is a serine protease zymogen consisting of 278 residues, which is homologous to horseshoe crab factor B.Subunit alpha, on the other hand, is a new type of mozaic protein consisting of 654 residues with intriguing features. The NH_2-terminal portion of this subnit is similar to bacterial beta-1,3-glucanases. Its 126-amino-acid COOH-terminus exhibits a repetitive sequence having partial homology to xylanases. Between these regions there are three repeating units of 47 amino acids, whose similarity to carbohydrate binding proteins. These may be the (1*3) -beta-D-glucan binding domain (s) of factor G.Thus, factor G,the primary initiator of the (1*3) -beta-D-glucan-sensitive coagulation pathway in the horseshoe crab, is a structurally unique heterodimeric serine protease zymogen and as such represents a new class of active defense proteins. Less
鲎血细胞裂解物对(1*3)-β-D-葡聚糖产生反应,启动酶级联反应,最终形成凝块。我们已经将丝氨酸蛋白酶酶原G因子纯化至均一,G因子直接被(1*3)-D-葡聚糖激活,并启动血淋巴凝血级联反应。G因子是由两个非共价缔合的亚基α(72 kDa)和β(37 kDa)组成的异二聚体蛋白。在存在(1*3)-D-葡聚糖(如凝胶多糖和副淀粉)的情况下,G因子被自催化活化为活性丝氨酸蛋白酶,称为G因子。这种活化伴随着两个亚基中Arg-X键的有限蛋白水解:72-kDa亚基α裂解为55-kDa和17-kDa片段,37-kDa亚基β缩短为34-kDa。使用参与血淋巴凝血级联反应的纯化蛋白质进行的重建实验表明,G因子能够直接激活凝血酶原,导致凝血酶的转化。 ...更多信息 Gen to coagulin gel.对这两种亚基的cDNA的分析表明,这些亚基来自不同的mRNA种类,因此由不同的基因编码。β亚基是一种丝氨酸蛋白酶酶原,由278个氨基酸残基组成,与鲎因子B同源。α亚基是一种新的镶嵌蛋白,由654个氨基酸残基组成,具有有趣的特征。该亚基的NH_2-末端部分与细菌β-1,3-葡聚糖酶相似。其126个氨基酸的COOH-末端显示出与木聚糖酶具有部分同源性的重复序列。在这些区域之间有三个47个氨基酸的重复单元,其类似于碳水化合物结合蛋白。这些可能是G因子的(1*3)-β-D-葡聚糖结合结构域。因此,G因子,马蹄蟹中(1*3)-β-D-葡聚糖敏感性凝血途径的主要起始物,是结构独特的异二聚体丝氨酸蛋白酶酶原,因此代表了一类新的主动防御蛋白。少

项目成果

期刊论文数量(36)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
岩永,貞昭: "ビタミンK-医学・生物学領域における新展開" 岩永,貞昭、斎藤,英彦、松田,道生、メディカル・ジャーナル社, 495頁 (1994)
Iwanaga, Sadaaki:“维生素 K - 医学和生物领域的新发展” Iwanaga、Sadaaki、Saito、Hidehiko、Matsuda、Michio,Medical Journal Inc.,495 页(1994 年)
  • DOI:
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  • 影响因子:
    0
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  • 通讯作者:
Yoshiki Miura: "Preparation and Properties of Monoclonal Antibodies against Lipopoly saccharide-Sensitive Serine Protease Zymogen,Factor C,from Horseshoe Crab(Tachypleustridentatua)Hemocytes." J.Biochim.112. 476-481 (1992)
Yoshiki Miura:“针对来自鲎血细胞的脂多糖敏感丝氨酸蛋白酶酶原、C 因子的单克隆抗体的制备和特性。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Hiroyuki Takeya: "Coahulation Factor X Activating Enzyme from Russill's Viper Venom(RVV-X):A Novel Metalloproteinase with Disintegrin(platelet aggregation inhibitor)-like and C-type Lectin-like Domaims." J.Biol.Chem.267. 14109-14117 (1992)
Hiroyuki Takeya:“来自罗西尔蝰蛇毒液的凝血因子 X 激活酶 (RVV-X):一种具有解整合素(血小板聚集抑制剂)样和 C 型凝集素样结构域的新型金属蛋白酶。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Yoshiki Miura: "A Horseshoe Crab Intracellular Coagulation Inhibitor with Characteristic of Serine Protease Inhibitor Superfamily:Its Purification,Property and cDNA Cloning." J.Biol.Chem.269. 542-547 (1994)
Yoshiki Miura:“一种具有丝氨酸蛋白酶抑制剂超家族特征的鲎细胞内凝血抑制剂:其纯化、性质和 cDNA 克隆。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Hitoshi Nishimura: "Human Factor X Has a Tetrasaccharide O-Glycosidically Linked to Serine-61 through the Fucose Residue." J.Biol.Chem.267. 17520-17525 (1992)
Hitoshi Nishimura:“人类因子 X 的四糖 O-糖苷通过岩藻糖残基与丝氨酸 61 连接。”
  • DOI:
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  • 期刊:
  • 影响因子:
    0
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IWANAGA Sadaaki其他文献

IWANAGA Sadaaki的其他文献

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{{ truncateString('IWANAGA Sadaaki', 18)}}的其他基金

Basic studies on Development of Anti-thrombotic Agents
抗血栓药物开发的基础研究
  • 批准号:
    04557015
  • 财政年份:
    1992
  • 资助金额:
    $ 17.28万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Molecular Mechanism of Extrinsic Blood Coagulation Pathway
外源性凝血途径的分子机制
  • 批准号:
    03044113
  • 财政年份:
    1991
  • 资助金额:
    $ 17.28万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Studies on the Activity Measurement for Blood Proteases using their Monoclonal Antibodies
用单克隆抗体测定血液蛋白酶活性的研究
  • 批准号:
    02557016
  • 财政年份:
    1990
  • 资助金额:
    $ 17.28万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Mechanism of Hemolymph Coagulation System in Invertebrates
无脊椎动物血淋巴凝固系统的机制
  • 批准号:
    02454539
  • 财政年份:
    1990
  • 资助金额:
    $ 17.28万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Initiation Mechanism of Extrinsic Blood Coagulation System
外源性凝血系统的启动机制
  • 批准号:
    63044110
  • 财政年份:
    1988
  • 资助金额:
    $ 17.28万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Development of Synthetic Fluorogenic Peptide Substrates for Blood Clotting Proteases
凝血蛋白酶合成荧光肽底物的开发
  • 批准号:
    63870017
  • 财政年份:
    1988
  • 资助金额:
    $ 17.28万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research
Hemolymph Coagulation and Defence Systems in Invertebrate Animals
无脊椎动物的血淋巴凝固和防御系统
  • 批准号:
    62480453
  • 财政年份:
    1987
  • 资助金额:
    $ 17.28万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Development and Application of Fluorogenic Peptide Substrates for Determination fo Blood Clotting Proteases
凝血蛋白酶测定荧光肽底物的研制及应用
  • 批准号:
    61880016
  • 财政年份:
    1986
  • 资助金额:
    $ 17.28万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research
Studies on Molecular Abnormality of Blood Coagulation and Fibrinolytic Factors
凝血及纤溶因子分子异常研究
  • 批准号:
    60480497
  • 财政年份:
    1985
  • 资助金额:
    $ 17.28万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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