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Development of enzyme-linked immunosorbent assay as a liver function test

开发酶联免疫吸附测定法作为肝功能测试

基本信息

批准号：
04557016
负责人：
MORI Masataka
金额：
$ 11.26万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

We developed a sensitive enzyme-linked immunosorbent assay (ELISA) for serum ornithine carbamoyltransferase (OCT) protein, and examined serum OCT concentrations in patients with various liver diseases. OCT concentrations were markedly elevated in cases of hepatic encephalopathy, "acute on chronic", and those with the acute phase of acute hepatitis, moderately in chronic hepatitis, Iiver cirrhosis, hepatocellular carcinoma, primary biliary cirrhosis, and slightly in those with a fatty Iiver. High percentages (92-94%) of patients with chronic hepatitis , liver cirrhosis and hepatocellular carcinoma had higher than normal concentration of serum OCT protein. There was a close correlation with aspartateaminotransferase (AST) and alanine aminotransferase (ALT) activities and moderate correlations with those of mitochondrial AST,glutamate dehydrogenase and g-glutamyl transpeptidase. The OCT/ALT ratio was higher in patients with liver cirrhosis than in those with chronic hepatitis (P<0.001), and was still higher in cases of hepatocellular carcinoma (P<0.05). In two patients with "acute on chronic", OCT concentrations decreased similarly with or more rapidly than AST or ALT activities after admission. In two patients with hepatic encephalopathy, the OCT concentrations changed similarly with AST and ALT activities. This OCT ELISA system will aid in diagnosing various liver diseases and for follow of the patients, and the OCT/ALTratio may serve for a differential diagnosis of liver diseases.

我们开发了一种灵敏的酶联免疫吸附试验（ELISA）来检测血清鸟氨酸氨基甲酰转移酶（OCT）蛋白，并检测了各种肝病患者的血清OCT浓度。 OCT 浓度在肝性脑病、“急性加慢性”以及急性肝炎急性期患者中显着升高，在慢性肝炎、肝硬化、肝细胞癌、原发性胆汁性肝硬化患者中中度升高，在脂肪肝患者中轻度升高。慢性肝炎、肝硬化和肝细胞癌患者中高比例（92-94%）的血清 OCT 蛋白浓度高于正常浓度。与天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)活性密切相关,与线粒体AST、谷氨酸脱氢酶、g-谷氨酰转肽酶活性中度相关。肝硬化患者OCT/ALT比值高于慢性肝炎患者（P<0.001），肝细胞癌患者OCT/ALT比值更高（P<0.05）。在两名“急性加慢性”患者中，入院后 OCT 浓度下降速度与 AST 或 ALT 活动相似或更快。在两名肝性脑病患者中，OCT 浓度随 AST 和 ALT 活性的变化类似。该OCT ELISA系统将有助于诊断各种肝脏疾病和跟踪患者，OCT/ALTratio可用于肝脏疾病的鉴别诊断。