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Gene cascades in cell differentiation and plasticity

细胞分化和可塑性中的基因级联

基本信息

批准号：
09044323
负责人：
MORI Masataka
金额：
$ 4.42万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

There are three isoforms of nitric oxide synthase (NOS), neuronal nNOS, inducible iNOS and enclotherial eNOS.eNOS and nNOS are expressed constitutively in CNS and are involved in neuronal activities and neuronal degenerative diseases. Regional distribution of neurons expressing nNOS mRNA in the rat brain was examined by non-radioisotope in situ hybridization, using digoxigenin-labeled complementary RNA probes. Clustering of intensely positive neurons was observed in discrete areas including the main and accessory olfactory bulbs, the islands of Calleja, the amygdala, the paraventricular nucleus of the thalamus, several hypothalamic nuclei, the lateral geniculate nucleus, the magnocellular nucleus of the posterior commissure, the superior and inferior colliculi, the laterodorsal and pedunculopontine tegmental nuclei, the nucleus of the trapezoid body, the nucleus of the solitary tract and the cerebellum. Strongly-stained isolated neurons were scattered mainly in the cerebral cortex, the basal ganglia and the brain stem, especially the medulla reticular formation. In the hyppocampus, an almost uniform distribution of moderately stained neurons was observed in the granular cell layer of the dentate gyrus and in the pyramidal cell layer of the ammon's horn, while more intensely stained isolated neurons were scattered over the entire hippocampal region. These observations can serve as a good basis for studies on function and gene regulation of neuronal nitric oxide synthase.

一氧化氮合酶（nitricoxidesynthase，NOS）有三种亚型，即神经元型nNOS、诱导型iNOS和神经元型eNOS，eNOS和nNOS在中枢神经系统中组成性表达，参与神经元活动和神经元退行性疾病。用地高辛标记的互补RNA探针，用非放射性同位素原位杂交方法研究了大鼠脑内表达nNOS mRNA的神经元的区域分布。在主、副嗅球、Calleja岛、杏仁核、丘脑室旁核、下丘脑多个核团、外侧膝状体核、后连合大细胞核、上级、下丘、被盖背外侧核、脚桥核、斜方体核、孤束核和小脑。强染色的神经元主要散在分布于大脑皮质、基底节和脑干，尤以髓质网状结构为著。在海马，几乎均匀分布的中度染色的神经元中观察到的颗粒细胞层的齿状回和在锥体细胞层的阿蒙的角，而更强烈的染色孤立的神经元分散在整个海马区。这些观察结果为研究神经元型一氧化氮合酶的功能和基因调控奠定了良好的基础。