Development of Enzyme-Linked Immunosorbent Assays for Diagnosis of Liver Diseases
用于诊断肝病的酶联免疫吸附测定法的开发
基本信息
- 批准号:07557201
- 负责人:
- 金额:$ 2.75万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (A)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Urea cycle is composed of five enzymes, carbamylphosphate synthetase (CPS), ornithine transcarbamylase (OTC), argininosuccinate synthetase (AS), argininosuccinate lyase (AL) and arginase. All these enzymes are expressed most strongly in the liver. In addition to the liver, CPS and OTC are expressed moderately in the small intestine, and AS and AL are expressed moderately in the kidney. Among the five enzymes, CPS and OTC are mitochondrial, whereas AS,AL and arginase are cytosoloc . CPS consists 4-5% of total liver protein and other enzymes do 0.1-0.2%. We developed a sensitive enzyme-linked immunosorbent assay (ELISA) of OTC and showed that this system will and in diagnosing various liver diseases. In addition to previously prepared antibodies against OTC and CPS,we prepared antibodies against AS,AL and arginase. Using these antibodies, we analyzed intrahepatic localization of these enzymes. Among the five enzymes, CPS,AS,AL and arginase showed a strong peritortal to pericentral zonation, whereas OTC showed only a very weak zonation. These imformations will be important for interpretation of the ELISA data for the urea cycle enzymes in the plasma of patients.
尿素循环由氨甲酰磷酸合成酶(CPS)、鸟氨酸转氨甲酰酶(OTC)、氨基琥珀酸合成酶(AS)、氨基琥珀酸裂解酶(AL)和氨基甲酸酯酶五种酶组成。所有这些酶在肝脏中表达最强烈。除肝脏外,CPS和OTC在小肠中中度表达,AS和AL在肾脏中中度表达。在这5种酶中,CPS和OTC是线粒体酶,而AS、AL和ATP酶是胞浆酶。CPS占肝脏总蛋白的4-5%,其他酶占0.1- 0.2%。本文建立了一种灵敏的土霉素酶联免疫吸附试验(ELISA),并证明该方法可用于多种肝病的诊断。除了先前制备的针对OTC和CPS的抗体之外,我们还制备了针对AS、AL和抗凝血酶的抗体。使用这些抗体,我们分析了这些酶的肝内定位。在这五种酶中,CPS、AS、AL和乙酰胆碱酯酶表现出较强的从门周到中心周的带状分布,而OTC仅表现出很弱的带状分布。这些信息对于解释患者血浆中尿素循环酶的ELISA数据将是重要的。
项目成果
期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
森正敬: "酵素免疫測定法を用いた肝機能検査法の開発" 臨床化学. 26・3. 410- (1997)
森正孝:“使用酶免疫分析法的肝功能检测方法的开发”临床化学26・3。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Watanabe,Y.et al.: "A sensitive enzyme-linked immunosorbent assay of serum ornithine carbamoyltransferase" Enzyme & Protein. 48. 10-17 (1995)
Watanabe,Y.et al.:“血清鸟氨酸氨基甲酰转移酶的灵敏酶联免疫吸附测定”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Mori, M.: "Development of Enzyme-Linked Immunosorbent Assays for Diagnosis of Liver Diseases." Rinshokagaku. 26(3). 41c (1997)
Mori, M.:“用于诊断肝病的酶联免疫吸附测定的开发。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Dingemanse,M.A.,et al.: "Development of the ornithine cycle in rat liver : Zonation of a metabolic pathway" Hepatology. 24. 407-411 (1996)
Dingemanse,M.A. 等人:“大鼠肝脏中鸟氨酸循环的发展:代谢途径的分区”肝病学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Shiojiri, N.et al: "Mozaic pattern of ornithine transcarbaylase expression on spf-ash mouse liver." American Journal of pathology. 151(2). 413-421 (1997)
Shiojiri, N.等人:“spf-ash 小鼠肝脏上鸟氨酸转氨酶表达的马赛克模式。”
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- 影响因子:0
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MORI Masataka其他文献
MORI Masataka的其他文献
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{{ truncateString('MORI Masataka', 18)}}的其他基金
Regulation of nitric oxide (NO) synthesis and NO-induced apoptosis
一氧化氮 (NO) 合成和 NO 诱导的细胞凋亡的调节
- 批准号:
14370047 - 财政年份:2002
- 资助金额:
$ 2.75万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Regulation of NO synthesis by the urea cycle enzymes
尿素循环酶对 NO 合成的调节
- 批准号:
10557020 - 财政年份:1998
- 资助金额:
$ 2.75万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Studies of mitochondrial protein import factors in mammals
哺乳动物线粒体蛋白输入因子的研究
- 批准号:
10470034 - 财政年份:1998
- 资助金额:
$ 2.75万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Gene cascades in cell differentiation and plasticity
细胞分化和可塑性中的基因级联
- 批准号:
09044323 - 财政年份:1997
- 资助金额:
$ 2.75万 - 项目类别:
Grant-in-Aid for international Scientific Research
Roles of molecular chaperones in cell function
分子伴侣在细胞功能中的作用
- 批准号:
09276103 - 财政年份:1997
- 资助金额:
$ 2.75万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas (A)
Molecular Mechanism of Mitoshondrial Protein Import in Higher Animals
高等动物线粒体蛋白输入的分子机制
- 批准号:
08457040 - 财政年份:1996
- 资助金额:
$ 2.75万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Protein import into mitochondria and its disorders
蛋白质进入线粒体及其疾病
- 批准号:
06454180 - 财政年份:1994
- 资助金额:
$ 2.75万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Protein import into mitochondria and its disorders
蛋白质进入线粒体及其疾病
- 批准号:
04454174 - 财政年份:1992
- 资助金额:
$ 2.75万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Development of enzyme-linked immunosorbent assay as a liver function test
开发酶联免疫吸附测定法作为肝功能测试
- 批准号:
04557016 - 财政年份:1992
- 资助金额:
$ 2.75万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
Transport of Proteins into Mitochondria
将蛋白质转运至线粒体
- 批准号:
02454147 - 财政年份:1990
- 资助金额:
$ 2.75万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)