Roles of molecular chaperones in cell function
分子伴侣在细胞功能中的作用
基本信息
- 批准号:09276103
- 负责人:
- 金额:$ 216.96万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research on Priority Areas (A)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Cytosolic hsp70-dj1/dj2 chaperone system was found to prevent NO-induced apoptosis in macrophages. Knockout mice for the HSP47 gene were generated and characterized. Many mutants of SecA were isolated and their interaction with SecY was studied. A cytosolic factor which induces cytochrome c release depending on staurosporin and dATP, was purified and found to be 20S proteasome. Chaperone activity of mitochondrial hsp70 was analyzed using denatured luciferase as a substrate. Exogenously added amyloid fibers were found to induce fiber formation of endogenous amyloid proteins. Oxidation of thiol of newly-synthesized proteins was shown to occur through S-S shuffling with glutathione and cysteine. Adjucent molecule of FtsH, an AAA ATPase, was shown to be important for ATP hydrolysis. Haclp, a transcription factor involved in unfolded protein response (UPR) in yeast, was shown to be induced when its mRNA undergoes stress-specific splicing.
发现胞浆hsp 70-dj 1/dj 2分子伴侣系统可阻止NO诱导的巨噬细胞凋亡。产生并表征HSP 47基因的敲除小鼠。分离了许多SecA突变体并研究了它们与SecY的相互作用。一种依赖于星形孢菌素和dATP诱导细胞色素c释放的胞质因子被纯化,并被发现是20 S蛋白酶体。以变性荧光素酶为底物分析线粒体hsp 70的分子伴侣活性。外源性添加的淀粉样纤维被发现诱导内源性淀粉样蛋白的纤维形成。新合成的蛋白质的巯基氧化被证明是通过谷胱甘肽和半胱氨酸的S-S改组发生的。FtsH是一种AAA ATP酶,它的一个小分子对ATP水解有重要作用。Haclp是酵母中参与未折叠蛋白反应(unfolded protein response,UPR)的转录因子,其mRNA在胁迫条件下发生特异性剪接时被诱导表达。
项目成果
期刊论文数量(71)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Koide, T., et al.: "Substrate recognition of the collagen-specific molecular chaperone.HSP47:Structural requirement and binding regulation"J.Biol.Chem.. 274. 34523-34526 (1999)
Koide, T., et al.:“胶原特异性分子伴侣的底物识别.HSP47:结构要求和结合调节”J.Biol.Chem.. 274. 34523-34526 (1999)
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Esaki,M.,Rospert,S.,et al.: "Two distinct mechanisms drive ptrotein translocation across the mitochondrial outer membrane in the late step of the cytochome b2 import pathway"Proc.Natl.Acad.Sci.USA. 96. 11770-11775 (1999)
Esaki, M., Rospert, S., et al.:“在细胞染色体 b2 输入途径的后期,两种不同的机制驱动蛋白质易位穿过线粒体外膜”Proc.Natl.Acad.Sci.USA。
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Komiya, T., et. al.: "Binding of mitochondrial precusor proteins to the cytoplasmic domains of the import receptors Tom70 and Tom20 is determined by cytoplasmic chaperones." EMBO J.16. 4267-4275 (1997)
小宫,T.,等。
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Komiya, T., et al.: "Interaction of mitochondrial targeting signal with acidic receptor domains along the protein import pathway : Evidence for the acid chain hypothesis"EMBO J.. 17. 3886-3898 (1998)
Komiya, T. 等人:“线粒体靶向信号与蛋白质输入途径中酸性受体结构域的相互作用:酸链假说的证据”EMBO J.. 17. 3886-3898 (1998)
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Gotoh, T. and Mori, M.: "Arginase I down-regulates NO production and prevents NO-mediated apoptosis in murine macrophage-derived RAW264. 7 cells"J. Cell Biol.. 144. 427-434 (1999)
Gotoh, T. 和 Mori, M.:“精氨酸酶 I 下调 NO 产生并防止小鼠巨噬细胞衍生的 RAW264.7 细胞中 NO 介导的细胞凋亡”J。
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MORI Masataka其他文献
MORI Masataka的其他文献
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{{ truncateString('MORI Masataka', 18)}}的其他基金
Regulation of nitric oxide (NO) synthesis and NO-induced apoptosis
一氧化氮 (NO) 合成和 NO 诱导的细胞凋亡的调节
- 批准号:
14370047 - 财政年份:2002
- 资助金额:
$ 216.96万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Regulation of NO synthesis by the urea cycle enzymes
尿素循环酶对 NO 合成的调节
- 批准号:
10557020 - 财政年份:1998
- 资助金额:
$ 216.96万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Studies of mitochondrial protein import factors in mammals
哺乳动物线粒体蛋白输入因子的研究
- 批准号:
10470034 - 财政年份:1998
- 资助金额:
$ 216.96万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Gene cascades in cell differentiation and plasticity
细胞分化和可塑性中的基因级联
- 批准号:
09044323 - 财政年份:1997
- 资助金额:
$ 216.96万 - 项目类别:
Grant-in-Aid for international Scientific Research
Molecular Mechanism of Mitoshondrial Protein Import in Higher Animals
高等动物线粒体蛋白输入的分子机制
- 批准号:
08457040 - 财政年份:1996
- 资助金额:
$ 216.96万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of Enzyme-Linked Immunosorbent Assays for Diagnosis of Liver Diseases
用于诊断肝病的酶联免疫吸附测定法的开发
- 批准号:
07557201 - 财政年份:1995
- 资助金额:
$ 216.96万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Protein import into mitochondria and its disorders
蛋白质进入线粒体及其疾病
- 批准号:
06454180 - 财政年份:1994
- 资助金额:
$ 216.96万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Protein import into mitochondria and its disorders
蛋白质进入线粒体及其疾病
- 批准号:
04454174 - 财政年份:1992
- 资助金额:
$ 216.96万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Development of enzyme-linked immunosorbent assay as a liver function test
开发酶联免疫吸附测定法作为肝功能测试
- 批准号:
04557016 - 财政年份:1992
- 资助金额:
$ 216.96万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
Transport of Proteins into Mitochondria
将蛋白质转运至线粒体
- 批准号:
02454147 - 财政年份:1990
- 资助金额:
$ 216.96万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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