DEVELOPMENT OF SERUM-FABP ASSAY AND IT'S CLINICAL APPLICATION

我们成功克隆了两个FABP家族的新成员。一种是来自肠道的I-15P，另一种是来自大鼠皮肤的c-FABP。所得的I-15P cDNA序列有127个氨基酸与从大鼠肠上皮中纯化的大鼠I-15P蛋白序列一致。Northern blot分析表明，在卵巢中也检测到相同的表达，提示I-15P或其同源蛋白可能参与了类固醇激素产生组织中的类固醇代谢。另一个新家族成员c-FABP与髓磷脂P2蛋白、脂肪细胞FABP和热FABP具有50%的同源性。我们开发了血清FABP的测定方法，并测试了血清FABP是否可作为损伤组织的标志物。测定肠缺血大鼠血清I-FABP水平。大鼠肠结扎后1、2、4、8小时，I-FABP水平迅速升高。暂时性闭塞30分钟后1小时血凝素升高，然后恢复正常。组织学研究支持坏死的吸附上皮细胞释放I-FABP。结果表明，I-FABP是肠缺血的一个有用的生化制造者，特别是在早期可逆期。对9例急性心肌梗死（AMI）患者的血清h - fabp检测结果显示，4例心卒中后1.5 h内的血清样品处于正常水平，5例心卒中后1.5 h以上的血清样品显示h - fabp水平升高。然而，在经皮冠状动脉腔内成形术（PTCA）治疗后6小时内，它比血清肌酸激酶MB（CK-MB）和肌酸磷酸激酶（CKB）达到最高水平要快。PTCA治疗后尿H-FABP的升高与尿CK-MB和CPK的升高有明显的对比，后者很少检测到。因此，血清H-FABP是一个有用的生化指标，用于估计PTCA治疗后的再循环，而不是心脏病发作。

We have succeeded in cloning of two FABP family new members. One is a I-15P from intestine and the other is c-FABP from rat skin. The deduced I-15P cDNA sequence of 127 amino acids was identical that of rat I-15P protein purified from rat intestinal epithelium. Northern blot analysis indicated that the same was deteceted in the ovary suggesting that I-15P or a homologous protein might in volved in the metabolism of steroids in steroid hormone-producing tissues. Another new family member, c-FABP exhibits 50 % identity to myelin P2 protein, adipocyte FABP and heat FABP.We have developed the assay methods of serum FABPs and tested whether serum FABP is available as a marker of damaged tissues. Serum I-FABP was measured in rats with intestinal ischemic condition. The I-FABP level increased rapidly at 1, 2, 4 and 8 hr after ligation of rat intestine. It also increased at 1 hr after a 30-min transient occlusion and then returned to a normal level. Histological studies supported I-FABP release from necrotic adsorptive epithelial cell. The results suggest that the I-FABP is a useful biochemical maker for intestinal ischemia, particularly in the early reversible phase. The serum H-FABP determinations performed on 9 patitents with acute myocardial infarct (AMI) demonstrated that 4 serum samples obtaining within 1.5 h after heart stroke were within normal level, whereas 5 serum samples obtained with more than 1.5 h after heart stroke were showed elevated H-FABP levels. However, it reached maximum levels faster than serum creatine kinase MB(CK-MB) and creatine phosphokinase (CKB) levels within 6 h after percutaneous transluminal coronary angioplasty (PTCA) treatment. The increase of urinary H-FABP after PTCA treatment is in a marked contrast to urinary CK-MB and CPK which has been scarcely detected. Thus, serum H-FABP is a useful biochemical marker for estimation of recirculation by PTCA treatment rather than the onset of heart stroke.