心血管疾病已成为我国乃至全球致死率第一的疾病，其中急性心肌梗死（AMI）发病凶险，实现其“快而准”的早期发现和诊断具有重要的意义。目前，心肌标志物心脏脂肪酸结合蛋白（H-FABP）和心肌肌钙蛋白I（cTnI）的即时检测灵敏度不高、准确度不够是AMI诊断亟待解决的关键问题。以低毒性的CuInS2荧光量子作为标记材料构建免疫层析即时检测体系在实现快速检测的同时，可有效提高检测的灵敏度和稳定性。本项目拟采用“间隔滴加，原位生长”方式精确调控壳层包覆以合成高亮度、高稳定的CuInS2核壳量子点，并结合多步二氧化硅包覆制备量子点微球，以提高基于非镉量子点的免疫层析即时检测体系的稳定性和灵敏度，构建H-FABP和cTnI的灵敏、稳定的联合即时检测新方法。这不仅有助于实现AMI的早期发现并降低死亡率，同时也将为非镉量子点成为含镉量子点的替代标记材料构建新型的体外诊断技术奠定重要的基础。

The mortality rate of cardiovascular disease is the highest in China and even in the world. Among the cardiovascular disease, acute myocardial infarction (AMI) is very dangerous and the most immediately life threatening. So it is of great clinical significance to realize the rapid and accurate diagnosis of AMI. The problems of low sensitivity and imprecision results for point-of-care testing (POCT) of heart-type fatty acid binding protein (H-FABP) and cardiac troponin I (cTnI) are the key bottlenecks that restrict the early detection of AMI. The lateral flow immunoassay based on CuInS2 quantum dots (QDs) can greatly improve the sensitivity and stability of detection while ensuring rapid detection. In this project, we intend to develop a sensitive, stable and simultaneous POCT method of the two biomarkers based low-toxic CuInS2 QDs as a label material. The highly stable, high quantum yield CuInS2 core/shell QDs is synthesized by precisely controlling the shell coating with the method of “interval drop addition, in situ growth", and microsphere encapsulating multiple CuInS2 QDs is obtained by multi-step silica coating, which will greatly improve the sensitivity and stability of POCT for H-FABP and cTnI. A novel simultaneous POCT method based cadmium-free QDs for detection of H-FABP and cTnI will be constructed, which not only can help for diagnosis and treatment of AMI and then reducing the mortality rate, but also can lay an important foundation for the cadmium-free QDs to become the promising candidates for cadmium-based QDs to construct a novel in vitro diagnostic technique.