Elucidating the interplay of PMF determinants in mitochondria and chloroplasts in folio

阐明叶绿体中线粒体和叶绿体中 PMF 决定因素的相互作用

• 批准号: 525342617
• 负责人: Professor Dr. Markus Schwarzländer
• 金额: --
• 依托单位: Arbeitsgruppe Plant Energy Biology
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/525342617?language=en
• 关键词: Elucidating; interplay; PMF; determinants; mitochondria

Mitochondria and chloroplasts have been shaping eukaryotic life as we know it. At the center of the function of both organelles is the proton motive force (PMF). The PMF connects redox chemistry of electron transport with ATP synthesis, but also acts as a regulation hub that determines the function and the makeup of the organelle. In plants and algae, the PMF of both mitochondria and chloroplasts are interconnected via the cellular metabolic network. While efficient photosynthesis requires chloroplast and the mitochondrial bioenergetics to work in concert, how exactly this interplay is achieved and underpinned in vivo remains insufficiently understood. In P07, we will elucidate how the subcellular dynamics of protons (H+), potassium ions (K+) and calcium ions (Ca2+) determine PMF characteristics in mitochondria and chloroplasts in vivo. Using a mature rosette leaf of Arabidopsis thaliana as a model, we will exploit a set of fluorescent protein-based biosensors and high-resolution imaging to generate an atlas of H+, K+ and Ca2+ in the subcompartments of the chloroplasts and the mitochondria in folio. The atlas will not only comprise three-dimensional molecular information in living leaf tissues, but also responses to different illumination regimes. It will provide a hitherto unique reference and will be used to further corroborate the significance of candidate proteins for H+, K+ and Ca2+ transport across the organelle membranes in determining PMF characteristics in folio. A selection of genetic mutants of candidate proteins of the mitochondria and the chloroplasts to set organelle H+, K+ and Ca2+ dynamics will be tested for their impact on organellar PMF characteristics in folio. The role of several of those players, such as KEA3 and NHD1, will be assessed in close collaboration within this consortium, while that of others such as the MCUs, MICU, UCP1 and AOX1a will be assessed based on the recent advances by our lab. The atlas approach and the mutants combined will allow to address the central question to what extent changes in the PMF characteristics in one organelle affect the other. The significance of altered PMF dynamics for photosynthetic metabolism, organelle ultrastructure, and whole plant performance will become accessible through collaboration with leading experts within the consortium. An advanced understanding of PMF regulation in vivo and the relative significance of specific candidate protein players in setting PMF characteristics will be established as a result.

正如我们所知，线粒体和叶绿体一直在塑造真核生命。两个细胞器功能的核心是质子动力（PMF）。 PMF 将电子传递的氧化还原化学与 ATP 合成联系起来，同时也充当决定细胞器功能和组成的调节中心。在植物和藻类中，线粒体和叶绿体的 PMF 通过细胞代谢网络相互连接。虽然有效的光合作用需要叶绿体和线粒体生物能协同工作，但这种相互作用在体内究竟是如何实现和支撑的仍不清楚。在 P07 中，我们将阐明质子 (H+)、钾离子 (K+) 和钙离子 (Ca2+) 的亚细胞动力学如何决定体内线粒体和叶绿体中的 PMF 特征。使用拟南芥成熟的莲座叶作为模型，我们将利用一套基于荧光蛋白的生物传感器和高分辨率成像来生成叶绿体和线粒体子区室中的 H+、K+ 和 Ca2+ 图谱。该图谱不仅包含活叶组织中的三维分子信息，而且还包含对不同光照条件的响应。它将提供迄今为止独特的参考，并将用于进一步证实候选蛋白对跨细胞器膜的 H+、K+ 和 Ca2+ 转运在确定对开的 PMF 特征中的重要性。将测试线粒体和叶绿体候选蛋白的一系列基因突变体，以设置细胞器 H+、K+ 和 Ca2+ 动力学，以了解它们对对开细胞器 PMF 特性的影响。其中几个参与者（例如 KEA3 和 NHD1）的作用将在该联盟内密切合作进行评估，而其他参与者（例如 MCU、MICU、UCP1 和 AOX1a）的作用将根据我们实验室的最新进展进行评估。图谱方法和突变体相结合将能够解决一个细胞器中 PMF 特征的变化在多大程度上影响另一个细胞器的核心问题。通过与联盟内领先专家的合作，将了解改变 PMF 动力学对光合代谢、细胞器超微结构和整个植物性能的重要性。由此将建立对体内 PMF 调节的深入理解以及特定候选蛋白质参与者在设定 PMF 特征中的相对重要性。