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Making of multi-tolerant plants against complexed acid-soil stresses by molecular modifications of membrane lipid layers

通过膜脂层的分子修饰制造针对复杂酸性土壤胁迫的多耐受植物

基本信息

批准号：
23380041
负责人：
WAGATSUMA Tadao
金额：
$ 12.06万
依托单位：
Yamagata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2011
资助国家：
日本
起止时间：
2011-04-01 至 2014-03-31
项目状态：
已结题

项目摘要

In order to lower the content of phospholipids and to increase that of sterols in roots, we made the new plants by modification using two different techniques, i.e., culturing method and molecular method: Al-sensitive rice cultivar Koshihikari grown previously under P-deficient conditions and transformed by the overexpressions of PAH (key gene to exchange phospholipids into galactolipids) and HMG (key gene to biosynthesize sterols). Al tolerance and low-Ca tolerance under low pH conditions of P-deficient Al-sensitive Koshihikari were significantly enhanced, and Al tolerance and low-P tolerance of the several lines of the transformed Koshihikari were also enhanced. Among several tens of rice cultivars, low-P tolerant rice cultivar was found out to have a specific characteristics on the decomposition of P-containing metabolites. Several genes contributing to P-recycling ability were identified together with each function.

为了降低根中磷脂的含量并增加甾醇的含量，我们使用两种不同的技术，即，培养方法和分子方法：Al敏感的水稻品种Koshihikari先前在P-缺乏条件下生长，并通过PAH（将磷脂交换成半乳糖脂的关键基因）和HMG（生物合成甾醇的关键基因）的过度表达转化。缺铝敏感越光耐铝性和低pH条件下的低钙耐性显著增强，转化越光的几个株系耐铝性和低磷耐性也得到增强。在几十个水稻品种中，耐低磷水稻品种对含磷代谢产物的分解具有独特的特点。几个基因有助于P-回收能力一起确定每个功能。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Principles, Application and Assessment in Soil Science

土壤科学原理、应用和评估

DOI：
发表时间：
2011
期刊：
影响因子：
0
作者：
Watanabe;T.;et al
通讯作者：
et al

HMG is a promising key gene for the enhancement of aluminum tolerance of rice via modulating membrane sterols in root-tip portion

HMG是一个有前途的关键基因，通过调节根尖部分的膜甾醇来增强水稻的铝耐受性

DOI：
发表时间：
2013
期刊：
影响因子：
0
作者：
Wagatsuma;T.;Watanabe;T.;Toyomasu;T.;Kuroda;M.;Muranaka;T.;Ohyama;K.;Ishikawa;A.;Usui;M.;Maejima;E.;Khan;M. S. H.;Tawaraya;K.;Koyama;H.
通讯作者：
H.

Phosphorus deficiency enhances aluminum tolerance of rice (Oryza sativa) by changing the physicochemical characteristics of root plasma membranes and cell walls