Development of Damaged DNA Markers for Prevention by Food Factors

点击翻译按钮获取中文摘要

Free radical has been associated with important pathophysiological events in a variety of diseases, drug toxicities, and traumatic or ischemic injuries. It has been postulated that free radicals generated during the process may be responsible for these effects because of their ability to damage cellular membranes, proteins, and DNA.We have developed a unique immunochemical procedure for quantifying 8-oxodeoxyguanosine (8-OH-dG) generated in the oxidized DNA.Using this method, we found that sesamisol glucoside decreased the level of 8-OH-dG and considerably inhibits the hepatotoxicity of rats treated with carbon tetrachloride. Furthermore, we developed another immunochemical procedure for quantifying a cytotoxic aldehyde 4-hydroxy-2-nonenal (HNE) originated from the peroxidation of polyunsaturated fatty acide. We characterize the specificity of this assay and found that considerable amount of HNE is generated in the kidney of rats treated with a renal carcinogen ferric nitrilotriacetate. Recently, we have obtained a direct evidence that oxidatively modified low density lipoproteins (LDL) also possess a considerable amount of HNE that is capable of reacting with other molecules.