Chemistry and functions of novel type of petide antimutagenic factors produced by microorganisms

微生物产生的新型肽类抗突变因子的化学和功能

• 批准号: 05660116
• 负责人: OSAWA Toshihiko
• 金额: $ 1.34万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660116/
• 关键词: Antimutagenic factor; Streptomyces; Peptide; Antimutagens; E.coli; E.coli MP-1; E.coli WP2s; 微生物代謝産物; 大腸菌MP-1

In order to obtain novel type of antimutagenic factors produced by Streptomyces sp.using E.coli MP-1 and WP2s strains as the bioassay systems. Purification and isolation of active compound by column chromatogrphy and preparative HPLC from the metabolites of Streptomyces SP AJ 9455, two main antimutagenic compounds were isolated. One of antimutagenic components, BA-2, has been identified as N-methyl-valyl-amycleomycin. Antimutagenic mechanism has been investigated using several E.coli strains and it was found that BA-2 has strong inhibitory effects on SOS mutagenesis. Other antimutagenic factor which was named BA-1 supposed to be polypeptide whose molecular weight is 7000-10000, however, BA-1 has been speculated as small molecule peptide type antimutagen. Structure analyzes have been carried out using NMR,MS,IR and also aminoacid analysis, and it was found BA-1 has molecular weight of 436 although other detailed structre is still unknown. Now, structure analyzes and investigation of antimutagenesis mechanism of BA-1 are underway.

以大肠杆菌MP-1和WP 2s菌株为生物测定系统，对链霉菌产生的新型抗突变因子进行筛选。用柱层析和制备HPLC从链霉菌AJ 9455代谢产物中分离纯化活性物质，从代谢产物中分离得到两种主要的抗突变活性物质。BA-2是抗突变成分之一，已被鉴定为N-甲基缬氨酰霉素。用几种大肠杆菌菌株研究了BA-2的抗突变机制，发现BA-2对SOS突变有很强的抑制作用。另一种抗突变因子BA-1被认为是分子量在7000-10000之间的多肽，但BA-1被推测为小分子肽类抗突变因子。用核磁共振、质谱、红外光谱和氨基酸分析等方法对BA-1进行了结构分析，确定其分子量为436，其它结构尚不清楚。目前，BA-1的结构分析和抗突变机制的研究正在进行中。

项目成果

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