Development of a 3- dimensional culture model system simulating arterial intima and its application for atherosclerosis study.
模拟动脉内膜的三维培养模型系统的开发及其在动脉粥样硬化研究中的应用。
基本信息
- 批准号:05557016
- 负责人:
- 金额:$ 4.48万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Developmental Scientific Research (B)
- 财政年份:1993
- 资助国家:日本
- 起止时间:1993 至 1995
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1. Development of a 3-dimensional (3-D) culture system simulating arterial intimaThe culture system contains a layr of amnion membrane as an arterial intima. The methods for collecting, preparing and storing amnion membranes were studied. In addition, the system was further refined to facilitate convenient use. Several types of modified systems were made according to the different purposes.2. The properties of endothelial cells cultured in the 3-D culture systemCompared to endothelial cells cultured in conventiona plastic dish, the endothelial cells cultured in this system bear several properties similar to those endothelial cells in vivo, including the formation of tight junctions, expression of ZO-1 protein, and polarized secretion of endothelin-1.3. Application in atherosclerosis studyIt was found that this system is specifically useful in analyzing monocyte, lymphocyte and endothelial cell interactions, which is one of the earliest process in atherogenesis. We also found that plasm … More a LDL could be modified or oxidized by cultured endothelial cells through which, a fatty streak-like lesion can be produced in vitro when macrophages were co-present. We have attempted to study the influence of various kinds of cytokines such as interleukin-1 and tumor necrosis factor in the fatty streak formation using the current system. To clarify the mechanisms involved in this process, highly purified monocytes, lymphocytes and their subpopulation are required. Currently, we are trying to use immno-magnet isolation method for this purpose.4. Application of the 3-D culture system for non-vascular models-Development of an organ model.A novel type of the 3-D culture system has been developed ; it consists of upper and lower compartments divided by a layr of amnion membrane. On each side of the membrane surface, different types of cells can be cultured separately. In this study, epithelial cells from tracheal mucosa are cultured on the upper surface and endothelial cell or fibroblasts on the lower at the same time. We found that it may be possible to use this 3-D culture system as a new organ model in the future.According the above results, custom-made 3-D culture system of arterial intima or modified 3-D systems for different aims can be easily available commercially from some vendors. We believe that this system is not only useful for the study of atherosclerosis but for the study of inflammation mechanism and cancer metastasis by molecular biology as well. Less
1.一种模拟动脉内膜的三维培养系统的建立该培养系统含有一层羊膜作为动脉内膜。研究了羊膜的收集、制备和保存方法。此外,该系统进一步完善,以方便使用。根据不同的工艺条件,制作了几种不同类型的改性体系.三维培养系统中培养的内皮细胞的特性与传统培养皿中培养的内皮细胞相比,三维培养系统中培养的内皮细胞具有与体内内皮细胞相似的特性,包括紧密连接的形成、ZO-1蛋白的表达以及内皮素-1的极化分泌。在动脉粥样硬化研究中的应用研究发现,该系统特别适用于分析单核细胞、淋巴细胞和内皮细胞的相互作用,这是动脉粥样硬化形成的最早过程之一。我们还发现 ...更多信息 α LDL可被培养的内皮细胞修饰或氧化,当巨噬细胞共存时,可通过其在体外产生脂肪条样病变。我们尝试使用现有的系统来研究各种细胞因子如白细胞介素-1和肿瘤坏死因子在脂肪条纹形成中的影响。为了阐明这一过程中涉及的机制,需要高度纯化的单核细胞,淋巴细胞及其亚群。目前,我们正在尝试使用免疫磁隔离方法来实现这一目的。三维培养系统在非血管模型中的应用-器官模型的建立一种新型的三维培养系统已被开发出来;它由一层羊膜分隔的上、下隔室组成。在膜表面的每一侧,可以分别培养不同类型的细胞。本研究采用气管粘膜上皮细胞在上表面培养,内皮细胞或成纤维细胞在下表面培养的方法。我们发现这种三维培养系统有可能作为一种新的器官模型,根据上述结果,定制的动脉内膜三维培养系统或针对不同目的的改进的三维培养系统可以很容易地从一些供应商处商业化获得。我们认为,该系统不仅对动脉粥样硬化的研究,而且对炎症机制和肿瘤转移的分子生物学研究也是有用的。少
项目成果
期刊论文数量(17)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Haraoka S., Shimokama T., Watanabe T.: "Participation of T lymphocytes in atherogenesis : sequential and quantitative observation of aortic lesions of rats with diet-induced hypercholesterolemia using en face double immunostaining" Virchows Archive. 426.
Haraoka S.、Shimokama T.、Watanabe T.:“T 淋巴细胞参与动脉粥样硬化形成:使用正面双重免疫染色对饮食诱导的高胆固醇血症大鼠的主动脉病变进行连续和定量观察”Virchows Archive。
- DOI:
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- 影响因子:0
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- 通讯作者:
Haraoka S.,et al.: "Participation of T lymphocytes in atherogenesis:sequential and quantitative observation of aortic lesions of rats with diet-induced hypercholesterolemia using en face double・・・・" Virchows Archive. 426. 307-315 (1995)
Haraoka S. 等人:“T 淋巴细胞参与动脉粥样硬化:使用 enface double 对饮食诱导的高胆固醇血症大鼠的主动脉病变进行连续和定量观察”Virchows Archive。 426. 307-315 (1995)
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- 影响因子:0
- 作者:
- 通讯作者:
Haraoka S., Shimokama T., Watanabe T.: "Morphological fate and sequelae of human atherosclerosis : Evaluation of immune mechanisms in atherosgenesis through immunohistological and ultrastructural analysis" Pathol International. 45. 801-814 (1995)
Haraoka S.、Shimokama T.、Watanabe T.:“人类动脉粥样硬化的形态命运和后遗症:通过免疫组织学和超微结构分析评估动脉粥样硬化中的免疫机制”Pathol International。
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- 影响因子:0
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Haraoka S.,et al.: "Morphological fate and sequelae of human atherosclerosis:Evaluation of immune mechanisms in atherosgenesis through immunohistological and ultrastructural analysis" Pathol International. 45. 801-814 (1995)
Haraoka S.,et al.:“人类动脉粥样硬化的形态命运和后遗症:通过免疫组织学和超微结构分析评估动脉粥样硬化中的免疫机制”Pathol International。
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- 影响因子:0
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渡辺 照男,他: "動脈硬化とマクロファージ/Tリンパ球" 血管と内皮. 4. 456-465 (1994)
Teruo Watanabe 等:“动脉粥样硬化和巨噬细胞/T 淋巴细胞”血管和内皮细胞。4. 456-465 (1994)。
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WATANABE Teruo其他文献
WATANABE Teruo的其他文献
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{{ truncateString('WATANABE Teruo', 18)}}的其他基金
Effects of MMP-9 on plaque rupture: development of a novel model for acute coronary syndromes.
MMP-9 对斑块破裂的影响:急性冠脉综合征新型模型的开发。
- 批准号:
23590401 - 财政年份:2011
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Investigation of disease-specific MMP to conquer acute coronary syndrome ?study with transgenic rabbit
疾病特异性MMP征服急性冠脉综合征的研究——转基因兔研究
- 批准号:
20590343 - 财政年份:2008
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Elucidation of MMP-12 functions in atherosclerosis using transgenic rabbit models
使用转基因兔模型阐明 MMP-12 在动脉粥样硬化中的功能
- 批准号:
13470046 - 财政年份:2001
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
DEVELOPMENT OF CLONE TRANSGENIC RABBITS FOR THE STUDY OF ATHEROSCLEROSIS
用于动脉粥样硬化研究的克隆转基因兔的开发
- 批准号:
11557016 - 财政年份:1999
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Role of apo (a) in atherogenesis. A study using transgenic rabbits expressing human apo (a)
apo (a) 在动脉粥样硬化形成中的作用。
- 批准号:
10470046 - 财政年份:1998
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Reconstruction of Early Paleozoic arc-trench system: a case study in the eastern Gondwana margin
早古生代弧沟系统的重建——以冈瓦纳大陆边缘东部为例
- 批准号:
10041102 - 财政年份:1998
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for Scientific Research (A).
Development of transgenic rabbits as models for atherosclerotic research
开发转基因兔作为动脉粥样硬化研究模型
- 批准号:
08557017 - 财政年份:1996
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Studies on Immunobiological Mechanisms in Atherogenesis
动脉粥样硬化形成的免疫生物学机制研究
- 批准号:
07457047 - 财政年份:1995
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Reconstruction of pre-breakup Paleozoic-suture of Gondowana continent in Australia
澳大利亚冈多瓦纳大陆裂解前古生代缝合带的重建
- 批准号:
04041012 - 财政年份:1992
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for international Scientific Research
Role of Macrophages and Immune Cells in Atherogenesis
巨噬细胞和免疫细胞在动脉粥样硬化形成中的作用
- 批准号:
04454181 - 财政年份:1992
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
相似海外基金
Research on activation and differentiation of airway epithelial cells by using of 3-dimensional culture system.
利用三维培养系统进行气道上皮细胞活化和分化的研究。
- 批准号:
07670655 - 财政年份:1995
- 资助金额:
$ 4.48万 - 项目类别:
Grant-in-Aid for Scientific Research (C)