Development of intravascular targeting hyperthermia using dextran magnetite complex

这是一个基础性的研究，以发展一种“血管内靶向热疗”，其中一个深坐的肿瘤是使用血管内管理的磁性材料与外部施加的交流磁场一起加热。作为发热材料，我们采用葡聚糖磁铁矿复合物（DM），这是一种“磁流体”。DM在AC磁场中的热产生比先前报道的多畴铁氧体颗粒高得多，这是由于不同的热产生机制。我们设计了两种含DM的栓塞材料，DM/碘油（] SY. [）乳液和DM/可降解淀粉微球（DM/DSM）悬浮液。体外实验表明，这些制剂中DM的产热能力没有降低。将这些材料注入日本白色家兔的肾动脉内，可实现选择性加热（10 ℃/10 min以上）。栓塞肾脏暴露于100 kHz AC磁场后， ...更多信息 5000 A/m。栓塞肾中的平均铁浓度为4.9（± SY）。2.4mg Fe/g组织。一个移植到兔子肝脏中的VX 2肿瘤的类似实验证明了肿瘤的充分加热。在目前含有栓塞材料的DM成分中，DM逃逸进入体循环，并在动脉内给药后6小时内被网状内皮系统（RES）捕获。此外，使用C3 H小鼠模型的实验显示，DM的产热能力随着其代谢而降低13 EA 01：这是开发“血管内靶向热疗”的基础研究，其中使用血管内施用的磁性材料连同外部施加的AC磁场加热深部肿瘤。作为发热材料，我们采用葡聚糖磁铁矿复合物（DM），这是一种“磁流体”。DM在AC磁场中的热产生比先前报道的多畴铁氧体颗粒高得多，这是由于不同的热产生机制。我们设计了两种含DM的栓塞材料，DM/碘油（] SY. [）乳液和DM/可降解淀粉微球（DM/DSM）悬浮液。体外实验表明，这些制剂中DM的产热能力没有降低。将这些材料注入日本白色家兔的肾动脉内，可实现选择性加热（10 ℃/10 min以上）。暴露于约15000 A/m的100 kHz AC磁场后栓塞肾脏的体积。栓塞肾中的平均铁浓度为4.9（± SY）。2.4mg Fe/g组织。一个移植到兔子肝脏中的VX 2肿瘤的类似实验证明了肿瘤的充分加热。在目前含有栓塞材料的DM成分中，DM逃逸进入体循环，并在动脉内给药后6小时内被网状内皮系统（RES）捕获。Moreover，an experime减

This is a fundamental study to develop an "intravascular targeting hyperthermia" , in which a deep seated tumor is heated using an intravascularly administered magnetic material together with an externally applied AC magnetic field. As a heat generating material, we employed dextran magnetite complex (DM) , which is a kind of 'magnetic fluid'. The heat production of DM in an AC magnetic field is much higher than previously reported multidomain ferrite particles due to a different mechanism of heat generation. We designed two DM-containing embolic materials, a DM/Lipiodol(]SY.encircledR.[) emulsion and a DM/degradable starch microsphere (DM/DSM) suspension. In vitro experiments showed that the heat generating capacity of DM was not reduced in these preparations. When these materials were injected into the renal arteries of Japanese white rabbits, they achieved selective heating (over 10ﾟC/10min.) of the embolized kidney following exposure to a 100kHz AC magnetic field of approximately 1 … More 5000 A/m. The average iron concentration in the embolized kidney was 4.9 (]SY.+-.[) 2.4mg Fe/g tissue. A similar experiment with a VX2 tumor transplanted in the liver of a rabbit demonstrated sufficient heating of the tumor. With current composition of DM containing embolic material, DM escapes into systemic circulation and is trapped by reticulo endothelial system (RES) within 6 hours of intraarterial administration. Moreover, an experiment using C3H mouse model revealed that the heat generating capacity of DM is decreased as it is metabolize13EA01 : This is a fundamental study to develop an"intravascular targeting hyperthermia" , in which a deep seated tumor is heated using an intravascularly administered magnetic material together with an externally applied AC magnetic field. As a heat generating material, we employed dextran magnetite complex (DM) , which is a kind of 'magnetic fluid'. The heat production of DM in an AC magnetic field is much higher than previously reported multidomain ferrite particles due to a different mechanism of heat generation. We designed two DM-containing embolic materials, a DM/Lipiodol(]SY.encircledR.[) emulsion and a DM/degradable starch microsphere (DM/DSM) suspension. In vitro experiments showed that the heat generating capacity of DM was not reduced in these preparations. When these materials were injected into the renal arteries of Japanese white rabbits, they achieved selective heating (over 10ﾟC/10min.) of the embolized kidney following exposure to a 100kHz AC magnetic field of approximately 15000 A/m. The average iron concentration in the embolized kidney was 4.9 (]SY.+-.[) 2.4mg Fe/g tissue. A similar experiment with a VX2 tumor transplanted in the liver of a rabbit demonstrated sufficient heating of the tumor. With current composition of DM containing embolic material, DM escapes into systemic circulation and is trapped by reticulo endothelial system (RES) within 6 hours of intraarterial administration. Moreover, an experime Less