Development of Bioreactor System for the Treatment of Chromate Wastewater and the Recycle of Chromium using Chromate Reducing Bacteria Enterobacter cloacae HO-1

利用铬酸盐还原菌阴沟肠杆菌HO-1处理铬酸盐废水及回收铬的生物反应器系统的开发

• 批准号: 05558074
• 负责人: FUJIE Koichi
• 金额: $ 10.24万
• 依托单位: Toyohashi University of Technology (1994-1995)Yokohama National University (1993)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05558074/
• 关键词: Hexavalent chromium containing wastewater; Chromate-resistant and chromate-reducing bacteria; Enterobacter cloacae strain HO-1; Bioreactor system; Mathematical model; Recycle of Chromium; Chromium ore residue; 6価クロム; 排水処理; 最適運転操作; 微生物動態解析; 水酸化ク

A novel bioreactor system was developed for the simultaneous treatment of toxic and mutagenic hexavalent chromium (chromate) and high strength organic pollutants by introducing chromate-resistant and chromate-refucing bacteria Enterobacter cloacae strain HO-1. Growth and chromate reducing characteristics of E.cloacae HO-1 were extensively investigated. To enrich the active E.cloacae HO-1 in the bioreactor, the acrated cultivation with oxidation-reduction potential (ORP) control was successfully applied. Characteristics of the bioreactor system were studied to optimize its operating condition. Based upon the growth and chromate reducing kinetics of HO-1, a mathematical model to simulate the change of chromate removal rate per unit volume of bioreactor and that per unit cell mass under the fed-batch operation was proposed. Effects of repeated cultivation of HO-1 with ORP-controlled acration and the cumulative chromate reduction perunit cell mass on the microbial activity were investigated to represent the kinetic expression of chromate reduction. By using the mathematical model developed through the present research, relationship between the operating conditions and the chromate reduction rate in the bioreactor weas simulated and the model was verified by comparing with the observed data. Thus the optimal operating conditions to give the higher reduction rate and the longer operating interval with and without actated cultivation of HO-1 were extensively discussed. This bioreactor can be used for the treatment of chromate wastewater provided that some high concentration organic wastes are available. The specific rate of chromate reduction is as high as 500 mg-chromate/g-microbes.The rate of chromate reduction in the bioreactor is around 10-15 g-Cr^<6+>/m^3h. Chromium hydroxide, products of biological chromate reduction, can be recycled for green-color pigment after the incineration of the sludge removed from the effluent.

利用抗铬和还原铬细菌阴沟肠杆菌HO-1，建立了一种新型生物反应器系统，用于同时处理毒性和致突变性六价铬（铬酸盐）和高浓度有机污染物。对该菌的生长和铬酸盐还原特性进行了研究。为了在生物反应器中富集具有活性的阴沟肠杆菌HO-1，成功地应用了氧化还原电位（ORP）控制的强化培养。研究了生物反应器系统的特性，以优化其操作条件。基于HO-1的生长和铬酸盐还原动力学，建立了模拟补料分批操作条件下单位体积生物反应器和单位细胞质量铬酸盐去除率变化的数学模型。研究了HO-1在ORP控制下的重复培养和单位细胞质量的累积铬酸盐还原量对微生物活性的影响，以表征其铬酸盐还原动力学的表达。利用所建立的数学模型，模拟了反应器中操作条件与铬酸盐还原速率的关系，并与实测数据进行了比较，验证了模型的正确性。因此，在有和无HO-1活化培养的情况下，获得更高还原率和更长操作间隔的最佳操作条件被广泛讨论。该生物反应器可用于处理含铬废水，只要有高浓度有机废水存在。比还原速率高达500 mg-Cr ~（6+）/g-微生物，反应器中的还原速率约为10-15 g-Cr ~（6+）/m^3·h。铬酸盐生物还原的产物氢氧化铬经焚烧后可回收用于绿色颜料。

项目成果

藤江幸一(児玉,他編): "還元細菌で有毒6価クロムを処理(「地球をまもる小さな生き物たち」の第3.4章)" 技報堂出版, 238 (1995)

Koichi Fujie（Kodama 等编辑）：“用还原细菌处理有毒的六价铬（《保护地球的小生物》第 3.4 章）” Gihodo Publishing，238（1995）

藤江幸一、他: "Development of Bioreactor System for the Treatment of Chromate Wastwater Using Enterobacter cloacae HO-1" Water Science and Technology. 30. 235-243 (1994)

Koichi Fujie 等人：“利用阴沟肠杆菌 HO-1 处理铬酸盐废水的生物反应器系统的开发”水科学与技术。 30. 235-243 (1994)

藤江幸一、他: "クロム含有排水の処理対策技術(I)クロムの利用とクロム含有排水の発生状況" 水処理技術. 34. 563-569 (1993)

Koichi Fujie等：“含铬废水的处理技术(I)铬的利用及含铬废水的产生状况”水处理技术34. 563-569(1993)。

Koichi Fujie,et al.: "Development of Bioreactor System for the Treatment of Chromate Wastwater Using Enterobacter cloacae HO-1" Water Science and Technology. 30. 235-243 (1993)

Koichi Fujie等人：“利用阴沟肠杆菌HO-1处理铬酸盐废水的生物反应器系统的开发”水科学与技术。

Hong-Ying Hu and Koichi Fujie: "Treatment and Recycle of Heavy Metal Wastewaters Using Biological Processes" Bunrigijutu (Separation Engineering). 25(2). 84-91 (1995)

Hong-Ying Hu 和 Koichi Fujie：“利用生物过程处理和回收重金属废水”Bunrigijutu（分离工程）。