DEVELOPMENT OF INTRAVITAL LASER MICROSCOPE FOR THE OBSERVATION OF OXYGEN DISTRIBUTION

也就是说，100多年来，微循环的主要功能之一是将氧气输送到组织，我们的理解是将氧气输送到组织中的过程被纳入了从血液到细胞的运输过程中。一个主要的问题是，我们不知道如何在各种组织中集中氧气的情况，特别是在血液容器和个人细胞周围的微生物中。在目前的研究中，我们使用了最近设计的磷酸盐显微镜，使其能够在组织的小区域内测量氧张力（pO 2），以检查组织周围的梯度pO 2。实验是在无定形的大鼠奶油蘑菇中进行的。Pd-porphyrin复合体，氧依赖性：磷酸酶染料曾用于测量问题中的五年曲线。pO 2是从衰变曲线的时间常数计算的，跟随Stern-Volmer equation ：到/t=1+Kq·到·pO 2到哪里，在O2的缺失中，在组织被测量和Kq是固定不变的。Obtained arteriolar pO2 values decreased according to increase the number of branching （1 st-order：70 mmHg，2 nd：55 mmHg，3 rd：45 mmHg）， however venular pO2 values at the different position showed almost same level（30 mmHg）。Tissue pO2 values showed large gradient between the area closed to arterioles （arteriolar pO2 minus 15- 20 mmHg）和100 mum apart （5- 8 mmHg）。它包含了一种现有的方法，该方法提供了一种连续的、非侵入性的氧气分布在显微镜长度尺度上。

Although it has been recognized for over 100 years that one of the principal functions of the microcirculation is to deliver oxygen to tissue, our understanding of the processes involved in the transport of oxygen from blood to cells is incomplete. A major problem is that we do not know how the concentration of oxygen in tissue varies, particularly in the vicinity of blood vessels and around individual cells. In the present study we used newly designed phosphorescence intravital microscopy, which enable to measure oxygen tension (pO2) in small regions of tissue, to examine the gradient of pO2 in the tissue around the arteriole. Experiments were performed in anesthetized rat cremaster muscle. Pd-porphyrin complexes, the oxygen-dependent : phosphorescence dye were used to measure the quenching decay curve in the tissue. The pO2 was calculated from the time constant of the decay curve, following the Stern-Volmer equation : to/t=1+Kq・to・pO2 where to and are the phosphorescence lifetimes in the absence of O2 and in the tissue being measured and Kq is the quenching constant. Obtained arteriolar pO2 values decreased according to increase the number of branching (1st-order : 70mmHg, 2nd : 55mmHg, 3rd : 45mmHg), however venular pO2 values at the different position showed almost same level(30mmHg). Tissue pO2 values showed large gradient between the area closed to arterioles (arteriolar pO2 minus 15-20mmHg) and 100mum apart (5-8mmHg) . It was concluded that present method offers the continuous, non-invasive determina-tion of oxygen distribution in the skeletal muscle on a microscopic length scale.