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Studies on the origin and evolution of tupai in Thailand

泰国图派的起源与演化研究

基本信息

批准号：
05041105
负责人：
NISHIDA Takao
金额：
$ 6.4万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

1.Six plasma loci, that is, PA,Alb, Pi, Gc, Tf and slow macroglobulin were screened, and PA,Alb, Gc and Tf loci showed polymorphism. The values of gene frequency of these loci was fluctuated in each locality, and genetic variability of plasma proteins of common tupai was higher than that of primate species. It is estimated that the degree of gene disversions within and between each locality is low.2.Restriction fragment length polymorphisms of mitochondrial DNA(mtDNA) were examined for the common tupai in order to investigate genetic variations within and between local populations, especially to test the significance of zoogeographical classification of twosubregions in the oriental faunal region. The degree of mtDNA differentiation was large between those populations of the Indochina region and the Malay Peninsula region, suggesting the significance of zoogeographical discrimination of two subregions in this species.3.The molecular analysis of the MHC class II cDNA of the common tree shrew, which might provide important informations on the evolution of the immune system in mammals, was carried out. Three clones (CL1, CL2 and CL3) with different nucleotide sequence were obtained and showed high homology indices to HLA class II DQ,DR and DP regions, respectively. Thus three isotypic forms of class II molecules, DP,DQ and DR,might exist in common tupai like in HLA system.4.The common tree shrew had well-developed cardiac myocyte layrs in the tunica media of the cranial vena cava. Musculature was distributed from the right atrium to the root of the subclavian vein. Because the Order Scandentia to which the common tree shrew belongs is a primitive group of mammalian orders, occurence of musculature in the cranial vena cava is suggested to have developed as a basic structure of the mammalian body.

1.筛选出PA、Alb、Pi、Gc、Tf和慢速巨球蛋白6个血浆位点，PA、Alb、Gc和Tf位点存在多态性。这些位点的基因频率值在各地区存在波动，普通土派的血浆蛋白遗传变异性高于灵长类。据估计，每个地方内部和之间的基因转移程度都很低。对常见土排的线粒体DNA（mtDNA）限制性内切片段长度多态性进行了分析，以探讨当地种群内和种群间的遗传变异，特别是对东方动物区两个亚区动物地理分类的意义进行了检验。印度支那地区和马来半岛地区的居群mtDNA分化程度较大，表明该种在动物地理上存在两个亚区区别的意义。本文对普通树鼩的MHCⅱ类cDNA进行了分子分析，为哺乳动物免疫系统的进化提供了重要信息。获得3个核苷酸序列不同的克隆CL1、CL2和CL3，分别与HLAⅱ类DQ区、DR区和DP区具有较高的同源性。因此，II类分子DP、DQ和DR三种同型形式可能与HLA系统一样存在于同一组中。普通树鼩在颅腔静脉中膜有发育良好的心肌细胞层。肌肉组织分布于右心房至锁骨下静脉根部。由于普通树鼩所属的Scandentia目是哺乳动物目的原始类群，因此颅腔静脉中肌肉组织的出现被认为是哺乳动物身体的基本结构。