Studies on the Biosynthesis of Natural Rubber

天然橡胶的生物合成研究

• 批准号: 05044029
• 负责人: OGURA Kyozo
• 金额: $ 3.84万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05044029/
• 关键词: Prenyltransferase; Natural Rubber; Farnesyl Diphosphate; Isopentenyl Diphosphate; Latex; Isomerase; Synthase; Isoprene Chain Elongation; 生合成; イソプレノイド; ポリプレノール; ゲラニルゲラニル二リン酸; ゴム伸長因子

Isopentenyl diphosphate (IPP) isomerase [EC 5.3.3.2], which catalyzes the reversible isomerization of IPP to dimethylallyl diphosphate, is presumed to be involved in rubber biosynthesis, but no direct evidence has been given for its occurrence in rubber latex. This enzyme activity was found in the C-serum powder prepared by lyophilization of centrifuged rubber latex of Hevea brasiliensis.In connection with the biosynthesis of rubber, prenyltransferase activities in the C-serum powder were examined. Farnesyl diphosphate (C_<15>) and geranylgeranyl diphosphate (C_<20>) were detected as major products. In addition, a polyprenyl diphosphate (-C_<30>) was obtained when geranyl diphosphate (C_<10>) was employed as the allylic substrate. When the C-serum was chromatographed on DEAE-Toyopearl, several prenyltransferase fractions were obtained. One of the partially purified enzyme fractions showed an activity of producing a polyprenyl product with a chain length longer than C_<80> as well as a C_<15> product.

异戊烯基二磷酸（IPP）异构酶[EC 5.3.3.2]催化IPP可逆异构化为二甲基烯丙基二磷酸，被认为参与橡胶生物合成，但没有直接证据表明其存在于橡胶胶乳中。从巴西橡胶树胶乳离心后冻干得到的C-乳清粉中发现了这种酶的活性，并检测了C-乳清粉中与橡胶生物合成有关的异戊烯基转移酶的活性。主要产物为法呢基二磷酸（C_<15>）和香叶基香叶基二磷酸（C_<20>）。此外，以香叶<30>基二磷酸（C_<10>）为烯丙基底物，得到了聚异戊二烯基二磷酸（-C_）。当C-血清在DEAE-Toyoprotein上层析时，得到几个异戊二烯基转移酶级分。部分纯化的酶组分之一显示出产生链长大于C_的聚异戊二烯产物<80>以及C_<15>产物的活性。

项目成果

T.Koyama,D.Wititsuwannakul,K.Asawatreratanakul,R.Wititsuwannakul,K.Ogura: "Isopentenyl Diphosphate Isomerase in Rubber Latex" Phytochemistry. (In press).

T.Koyama，D.Wititsuwannakul，K.Asawatreratanakul，R.Wititsuwannakul，K.Ogura：“橡胶乳胶中的异戊烯基二磷酸异构酶”植物化学。

K.Asawatreratanakul,T.Koyama,D.Wititsuwannakul,R.Wititsuwannakul,K.Ogura: "Characterization of Hevea Isopentenyl Diphosphate Isomerase" Proceedings of the 11th FAOBMB Symposium. (In press).

K.Asawatreratanakul、T.Koyama、D.Wititsuwannakul、R.Wititsuwannakul、K.Ogura：“橡胶树异戊烯基二磷酸异构酶的表征”第 11 届 FAOBMB 研讨会论文集。

H.Inoue,H.Sagami,T.Koyama,K.Ogoura: "Properties of Farnesol Phosphokinase of Botryococcus braunii" Phytochemistry. (In press).

H.Inoue，H.Sagami，T.Koyama，K.Ogoura：“布氏葡萄球菌法呢醇磷酸激酶的特性”植物化学。

H.Inoue,T.Korenga,H.Sagami,T.Koyama,K.Ogura: "Phosphoryration of Farnesol by a Cell-free System from Botryococcus Braunii" Biochem.Biophys.Res.Commun.200. 1036-1041 (1994)

H.Inoue、T.Korenga、H.Sagami、T.Koyama、K.Ogura：“来自布氏葡萄球菌的无细胞系统对金合欢醇的磷酸化”Biochem.Biophys.Res.Commun.200。

T.Koyama,S.Obata,M.Osabe,K.Saito,A.Takeshita,T.Nishino,K.Ogura: "Thermostable Farnesyl Diphosphate Synthase of Bacillus stearothermophilus:Crystallization and Site-directed Mutagenesis" Acta Biochim.Polonica.41. 281-292 (1994)

T.Koyama，S.Obata，M.Osabe，K.Saito，A.Takeshita，T.Nishino，K.Ogura：“嗜热脂肪芽孢杆菌的热稳定法尼基二磷酸合酶：结晶和定点诱变” Acta Biochim.Polonica.41