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Biosynthetic Studies of Natural Products and Their Application

天然产物生物合成研究及其应用

基本信息

批准号：
60303006
负责人：
OGURA Kyozo
金额：
$ 4.93万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

项目摘要

(1) Metabolic pathways for rishitin, a phytoalexin of potato were clarified, and hydrogen peroxide was identified as an endogeneous elicitor of this phytoalexin. (2) The absolute stereochemistry of new prenyltransferase reactions were established. (3) Essential activation factors for undecaprenyl diphosphate synthase were isolated and identified. (4) The two essential protein components of hexaprenyl diphosphate synthase were purified and their functions were characterized. (5) Bacterial squalene synthetase was first isolated. (6) Phosphoryl derivatives of ecdysteroids were identified as conjugates in Bombyx ovaries. (7) Biosynthesis of ursolic acid from aceatate in tissue cultures of Rabdasia japonica Hara was elucidated with ^<13>C,^2H-double labeled precursors. (8) A complex consisting of luciferase and luciferin derived pigment was isolated from Cypridina. (9) Stereo- and regeo-controlled reactions with NAD(P)H models were developed. (10) Stereoselective reductions by yeast were used to prepare useful chiral synthons. (11) Acromelic acids, agelasines, and bayunol were synthesized by effective methods. (12) New terpenoids with novel strucures were isolated from marine organisms. (13) New synthetic reactions useful for biosynthetic study were developed.Information was effectively exchanged among the members of this project to promote their research activities.

(1)阐明了马铃薯抗毒素rishitin的代谢途径，并确定过氧化氢是该植物抗毒素的内源激发子。(2)建立了新异戊二烯基转移酶反应的绝对立体化学。(3)十一异戊二烯基二磷酸合酶的关键激活因子的分离和鉴定。(4)纯化了六异戊二烯基二磷酸合酶的两个必需蛋白组分，并对其功能进行了表征。(5)细菌角鲨烯合成酶首先被分离出来。(6)蜕皮甾体的磷酰衍生物在卵巢中被鉴定为共轭物。(7)用^ C，^2 H-双标记前体化合物研究了香茶菜组织培养中熊果酸的生物合成<13>。(8)从Cypridina中分离到一种由荧光素酶和黄嘌呤衍生色素组成的复合物。(9)立体和regeo控制的反应与NAD（P）H模型的开发。(10)利用酵母菌的立体选择性还原反应制备了有用的手性配体。(11)采用有效的方法合成了丙烯醛酸、琼脂糖碱和白云醇。(12)从海洋生物中分离得到结构新颖的萜类化合物。(13)开发了对生物合成研究有用的新的合成反应。该项目的成员之间有效地交流了信息，促进了他们的研究活动。