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Conservation biology in plants common to Japan and China

日本和中国常见植物的保护生物学

基本信息

批准号：
06044160
负责人：
KONDO Katsuhiko
金额：
$ 9.54万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

In early August, 1996, Prof.Kuroki and Mr.Kokubugata went Jilin and Heilongjiang Provinces, China. Prof, Kuroki found some dioecious Rumex and Allium during their trip, and brought these live collections back to Japan for DNA conservation of Rumex. Mr.Kokubugata collected Dendranthema oreastra which is an endangered species in Mt.Chengbai, Jilin Province and performed molecular cytology for conservation of species diversity. Moreover, he sowed surface-sterilized seeds of this species on 1/2 MS agar medium in vitro. Prof.Shimada and Dr.Funamoto made a field trip to Sichuan Province, China in early August, 1996, and collected some species of Primula and Saxifraga. Prof.Shimada succeeded in in vitro conservation of genetic resource of Primula by tissue-culture from an explant of leaf. Dr.Funamoto cytologically analyzed species diversity of collected species. Prof.Kondo and Prof.Hizume made a field trip to Shaanxi Province, China in early October, 1996. Prof.Kondo collected some species of Chrysanthemum sensu lato, and performed molecular cytotaxonomy for analysis of species diversity. Moreover, he succeeded in vitro conservation of gene resources by tissue-culture. Prof.Hizume collected some species of Pinus, Picea, Abies and Ephedra and then, detected and analyzed their chromosome variation with fluorescent differential staining methods and fluorescent in situ hybridization.Dr.Zhou made a trip to Higashi-Hiroshima, Niigata, Ryotsu, Iwaki, Chiba and Nichinan, Japan with Dr.Funamoto for investigations and collections of Liliaceae and Ranunculaceae, in November and December, 1996, then, brought those live collections back to China. Moreover, he mastered techniques of plant tissue-culture in Laboratory of Plant Chromosome and Gene Stock, Faculty of Science, Hiroshima University. Prof.Hong made a trip to Higashi-Hiroshima, in February, 1997, and collected some species of Liliaceae and Ranunculaceae, and discussed further cooperative researches with Prof.Kondo.

1996年8月初，黑木教授和国深方先生前往中国吉林省和黑龙江省。黑木教授在旅途中发现了一些雌雄异株的酸模和葱属植物，并将这些活体标本带回日本进行酸模的DNA保存。作者采集了吉林省长白山濒危植物山菊，并对其进行了分子细胞学研究。此外，他还将该物种表面灭菌的种子播种在1/2 MS琼脂培养基上进行体外培养。岛田教授和船本博士于1996年8月初到中国四川省进行了实地考察，并收集了报春花和虎耳草属的一些物种。岛田教授以报春花属植物叶片为外植体，通过组织培养成功地进行了离体保存。船本博士对收集到的物种进行了物种多样性的细胞学分析。1996年10月初，近藤教授和希泽教授到中国陕西省进行了实地考察。近藤教授收集了部分野菊花属植物，并进行了分子细胞分类学研究。此外，他还成功地通过组织培养进行了基因资源的体外保存。1996年11月至12月，周博士随船本博士赴日本东广岛、新泻、凉津、磐城、千叶、日南等地进行百合科和毛茛科植物的调查和采集，并与日本学者进行了比较研究。把这些活标本带回中国在广岛大学理学部植物染色体与基因库研究室掌握植物组织培养技术。洪教授于1997年2月赴日本东广岛，采集了百合科和毛茛科的部分植物，并与近藤教授讨论了进一步的合作研究。