Chromosomal characterization of Japano-Chinese plants

日本-中国植物的染色体特征

• 批准号: 10044209
• 负责人: KONDO Katsuhiko
• 金额: $ 8万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044209/
• 关键词: Conservation biology; Endangered plant species of Japano-Chinese element; Conservation lines; Molecular cytogenetics; Molecular systematics; ISH; GISH; Flora of Japan; 染色体学; 日本; 中国; 野生植物; 絶滅危惧植物種; 染色体; 中華人民共和国; 保全

During the course of this three-year-grant-project form 1998-2000, we have studied on conservation and chromosomal characterization by molecular cytogenetics and molecular systematics in certain plant species common to Japan and China. We have visited and studied plant habitats in Jilin, Heilongjian, Nei Mongol Zizhiqu, Shaanxi, Gansu, Xinjiang Uygur Zizhiqu and Sichuan Provinces, China and have investigated and collected certain species and genera in the families of bryophytes, pteridophytes, Asteraceae (Compositae), Cyperaceae, Liliaceae, Orchidaceae, Ranunclaceae, Butulaceae, Hamamelidaceae, Pinaceae, Saxifragaceae, Rosaceae, Theaceae, Labiatae, Scrphulariaceae and so on for own researches. Those species were firstly counted their chromosome numbers and were, then, determined their karyotypes. Furthermore, we characterized those plants by in situ hybridization (ISH) including fluorescent in situ hybridization (FISH), genomic in situ hybridization (GISH), DNA sequencing, and so on. W … More e have successfully, very firstly marked by ISH using 5S rDNA certain super small chromosomes in woody species in three genera of the Betulaceae. This methodology may promise to analyze populations of woody plants which have commonly super small chromosomes. Multi-color FISH improvement in some members of the Pinaceae has been rapidly progressed. The most significant research progress has been made in Chrysanthemum sensu lato. Intergeneric hybridizations in various cross combinations were artificially made and immature embryos were harvested and rescued in tissue culture condition to obtain hybrid plants which were used for GISH.Thus, GISH in chrysanthemum hybrid chromosomes made it possible to discover new phenomena on somatic reciprocal translocations of chromosome segments, visualization and isolation of chromosomes in resting nuclei and arien chromosomes. These technologies may be useful for further investigations of chromosomes. Mosla, the Labiatae has been detected in molecular systematics and showed very interesting interspecific relationships among the Japano-Chinese species. Since all of the plant species collected during the project operation have been established cultivation lines and conserved for laboratory base, they would be investigated deeply, genetically and clarified and justified their systems in flora of Japan and China, and then, they would become important biological research materials. Less

在1998-2000年这个为期三年的资助项目期间，我们通过分子细胞遗传学和分子系统学对日本和中国常见的某些植物物种的保护和染色体特征进行了研究。我们对中国吉林省、黑龙江省、内蒙古紫枝区、陕西省、甘肃省、新疆维吾尔紫枝区和四川省的植物生境进行了考察和研究，调查并收集了苔藓植物、蕨类植物、菊科（菊科）、莎草科、百合科、 兰科、毛茛科、管科、金缕梅科、松科、虎耳草科、蔷薇科、山茶科、唇形科、玄参科等都有自己的研究。首先计算这些物种的染色体数目，然后确定其核型。此外，我们还通过原位杂交（ISH）对这些植物进行了表征，包括荧光原位杂交（FISH）、基因组原位杂交（GISH）、DNA测序等。 W … More e 首次使用 5S rDNA 通过 ISH 成功标记了桦木科三个属的木本物种中的某些超小染色体。这种方法有望分析通常具有超小染色体的木本植物种群。松科某些成员的多色 FISH 改良进展迅速。最重要的研究进展是在菊花意义上。人工进行各种杂交组合的属间杂交，收获未成熟胚并在组织培养条件下保存，获得用于GISH的杂交植株。因此，菊花杂交染色体的GISH使发现染色体片段的体细胞相互易位、静息核和染色体的可视化和分离等新现象成为可能。 外星人染色体。这些技术可能有助于染色体的进一步研究。 Mosla（唇形科）已在分子系统学中被发现，并显示出日本-中国物种之间非常有趣的种间关系。由于项目运营期间收集的所有植物物种均已建立栽培系并保存为实验室基地，将对其在日本和中国植物区系中进行深入的遗传学研究和阐明并论证其系统，从而成为重要的生物学研究材料。较少的

项目成果

Katsuhiko Kondo: "Chromosome studies in chrysanthemum flora of Taiwan. 1. Dendranthema horaimontana(Masam.) S.S.Ying and D.morii (Hayata) Kitam."Chromosome Srience. 3・1. 49-54 (1999)

Katsuhiko Kondo：“台湾菊花植物区系的染色体研究。1.Dendranthema horaimontana（Masam。）S.S.Ying 和 D.morii（Hayata）Kitam。”染色体 3・1（1999）。

Tsuneo Funamoto: "A cytological observation of Rodgersia aesculifolia Batalin (Saxifragaceae) collected in Shaanxi and Sichuan Provinces, China"Chromosome Science. 4(in press). (2000)

Tsuneo Funamoto：“中国陕西和四川省采集的Rodgersia aesculifolia Batalin（虎耳草科）的细胞学观察”染色体科学。

Katsuhiko Kondo: "Fluorescence genomic in situ hybridization identifies recipocal translocation of somatic chromosomes and origin of extra chromosome by an artificial, intergeneric hybrid between"Chromosome Science. 3. 15-19 (1999)

Katsuhiko Kondo：“荧光基因组原位杂交通过人工的属间杂交来识别体细胞染色体的相互易位和额外染色体的起源”染色体科学。

Kazuo Oginuma: "Chromosomal evolution of three genera in the Betulaceae concerning polyploidy revealed by in situ hybridization using 5S rDNA"Chromosome Science. 4・2(in press). (2000)

Kazuo Oginuma：“通过使用 5S rDNA 的原位杂交揭示了桦木科中三个属的染色体进化”，《染色体科学》4·2（出版中）。

Magdy Hussein Abd El-Twab: "Isolation of a particular chromosome of Ajania remotipinna in a chromosome complement of an artificial F_1 hybrid of Dendranthema lavandulifolia X Ajania remotipinna by use of genomic in situ hybridization"Chromosome Science. 3

Magdy Hussein Abd El-Twab：“通过使用基因组原位杂交，在 Dendranthema lavandulifolia X Ajania remotipinna 的人工 F_1 杂交体的染色体补体中分离 Ajania remotipinna 的特定染色体”《染色体科学》。