权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Hormonal Control of osmoregulation in euryhaline fis

广盐鱼渗透压调节的激素控制

基本信息

批准号：
06044270
负责人：
TAKEI Yoshio
金额：
--
依托单位：
Ocean Research Institute, University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Overseas Scientific Survey.
财政年份：
1994
资助国家：
日本
起止时间：
1994 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044270/
关键词：
Angiotensin Endothelin ANP vasotocin osmoregulation eel rainbow trout flounder

项目摘要

Takei visited Dr.Balment of Manchester University to conduct following experiments using flounder.(1) Papaverine, a vasorelaxant drug, was injected into the flounder, and the time course of drinking rate, plasma angiotensin II and vasotocin levels were measured. The effect of angiotensin II inhibition on drinking rate and plasma vasotocin level after papaverine was also examined.(2) We sequenced eel atrial natriuretic peptide (ANP) in eels for the first time in fishes. Synthetic eel ANP was injected into European eels, and the time course of change in drinking rate and plasma vasotocin level was examined.2. Hyodo also visited Dr.Balment and conducted the following experiments.(1) He found that the expression of vasotocin mRNA decreased after transfer of rainbow trout to seawater, and Dr.Balment found that plasma vasotocin level in the rainbow trout increased after transfer to seawater. Thus, they worked together to conduct similar experiments in the flounder.(2) The time course of change in vasotocin mRNA expression in the paraventricular nucleus of the trout brain was examined by Northern blot analysis.3. Hamano visited Dr.Hazon of University of St.Andrews and conducted the following experiments.(1) She had worked with Dr.Hazon previously for the vasopressor effect of angiotensin II in the dogfish, and found that mechanism of the pressor effect was quite different from that of other animals. She further analyzed the mechanism using an isolated aortic ring of dogfish.(2) She also examined the vasorelaxant activity of homologous C-type natriuretic peptide using isolated aortic ring of dogfish.

Takei拜访了曼彻斯特大学的Balment博士，利用比目鱼进行了如下实验。(1)将血管舒张药物罂粟碱注入比目鱼体内，测定其饮水率的时程、血浆血管紧张素II和血管收缩素水平。还检测了罂粟碱后抑制血管紧张素II对饮酒率和血浆缩宫素水平的影响。(2)我们首次在鱼类中对鳗鱼心房钠尿肽(ANP)进行了测序。将人工合成的鳗鱼ANP注射到欧洲鳗鱼体内，观察其饮水率和血浆催产素水平随时间的变化。 2． Hyodo还拜访了Balment博士并进行了以下实验。(1)他发现虹鳟鱼转移到海水中后血管催产素mRNA的表达下降，而Balment博士发现虹鳟鱼转移到海水中后血浆血管催产素水平升高。于是，他们合作在比目鱼身上进行了类似的实验。(2)采用Northern印迹分析法检测了鳟鱼大脑室旁核中血管催产素mRNA表达变化的时间过程。3． Hamano拜访了圣安德鲁斯大学的Hazon博士，并进行了以下实验。（1）她之前曾与Hazon博士合作研究血管紧张素II对角鲨的升压作用，发现升压作用的机制与其他动物有很大不同。利用分离的角鲨主动脉环进一步分析了其机制。(2)利用分离的角鲨主动脉环检测了同源C型利尿钠肽的血管舒张活性。