Hormonal Control of osmoregulation in euryhaline fis

竹井拜访了曼彻斯特大学的巴尔门特博士，用比目鱼进行了以下实验。(1)将血管舒张药罂粟碱注射到比目鱼体内，测定其饮水速率、血浆血管紧张素II和血管紧张素水平的时间进程。还检查了罂粟碱后血管紧张素II抑制对饮酒率和血浆血管紧张素水平的影响。(2)我们首次在鱼类中测定了鳗鱼心房钠尿肽（ANP）的序列。将人工合成的鳗鱼心钠素注射到欧洲鳗鲡体内，观察其饮水率和血浆血管生成素水平随时间的变化.兵藤还拜访了Balment博士，并进行了以下实验。(1)他发现将虹鳟鱼转移到海水中后，血管催产素mRNA的表达下降，巴尔门特博士发现虹鳟鱼转移到海水后血浆血管催产素水平升高。因此，他们一起在比目鱼身上进行了类似的实验。(2)采用北方印迹分析法检测鳟鱼脑室旁核内催产素mRNA表达的时程变化.滨野访问了圣安德鲁斯大学的哈松博士，并进行了以下实验。(1)她以前曾与哈松博士合作研究过角鲨中血管紧张素II的血管加压作用，发现加压作用的机制与其他动物的机制大不相同。她进一步分析了机制使用一个孤立的主动脉环的角鲨。(2)她还研究了同源C型利钠肽的血管舒张活性，使用分离的鲨鱼主动脉环。

Takei visited Dr.Balment of Manchester University to conduct following experiments using flounder.(1) Papaverine, a vasorelaxant drug, was injected into the flounder, and the time course of drinking rate, plasma angiotensin II and vasotocin levels were measured. The effect of angiotensin II inhibition on drinking rate and plasma vasotocin level after papaverine was also examined.(2) We sequenced eel atrial natriuretic peptide (ANP) in eels for the first time in fishes. Synthetic eel ANP was injected into European eels, and the time course of change in drinking rate and plasma vasotocin level was examined.2. Hyodo also visited Dr.Balment and conducted the following experiments.(1) He found that the expression of vasotocin mRNA decreased after transfer of rainbow trout to seawater, and Dr.Balment found that plasma vasotocin level in the rainbow trout increased after transfer to seawater. Thus, they worked together to conduct similar experiments in the flounder.(2) The time course of change in vasotocin mRNA expression in the paraventricular nucleus of the trout brain was examined by Northern blot analysis.3. Hamano visited Dr.Hazon of University of St.Andrews and conducted the following experiments.(1) She had worked with Dr.Hazon previously for the vasopressor effect of angiotensin II in the dogfish, and found that mechanism of the pressor effect was quite different from that of other animals. She further analyzed the mechanism using an isolated aortic ring of dogfish.(2) She also examined the vasorelaxant activity of homologous C-type natriuretic peptide using isolated aortic ring of dogfish.