Natriuretic peptides govern euryhalinity of fishes Analyses using transgenid technology

利钠肽控制鱼类的广盐度 使用转基因技术进行分析

• 批准号: 13304063
• 负责人: TAKEI Yoshio
• 金额: $ 26.12万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13304063/
• 关键词: natriuretic peptides; guanylins; adrenamedullins; molecular evolution; body fluid regulation; cardiovascular action; eel; puffer fish; ウナギ,Anguiila japonica; トラフグ,Takifugu rubripes; トランスジェニック技術; メダカ; 海水適応能; cDNAクローニング

Accumulating data from this laboratory strongly indicate that the natriuretic peptides (NPs) govern euryhalinity of eels ; atrial NP (ANP) promotes seawater adaptation and C-type NP (CNP) promotes freshwater adaptation. In this study, we attempted to determine that ANP is in fact essential for seawater adaptation using transgenic techniques. For this purpose, we use medaka fish that have plural akin species in the same genus that exhibit diverse osmotic adaptability and in which transgenic technique has already been established. Therefore, the production of ANP transgenic seawater medaka that cannot survive in seawater without transferred ANP gene will directly demonstrate the importance of ANP in seawater adaptation. However, ANP cDNA have not been cloned in medaka fish although cloning was successful in several other teleost species. Meanwhile, we could detect four CNPs, named CNP-1 through 4, and B-type NP (BNP) that had been thought as tetrapod-specific NP. Furthermore, we isolated CNP-4 type NPs from most primitive extant vertebrates, lamprey and hagfish. Therefore, we proposed new hypothesis of the molecular evolution of the NP family ; CNP-4 is an ancestral molecule of the NP family and it was diversified in bony fishes in parallel with the evolution of osmoregulatory mechanisms.

从这个实验室积累的数据强烈表明，利钠肽（NPs）管理宽盐度的鳗鱼，心房NP（ANP）促进海水适应和C型NP（CNP）促进淡水适应。在这项研究中，我们试图确定，心钠素实际上是必不可少的海水适应使用转基因技术。为此，我们使用在同一属中具有多个近缘种的青鳉鱼，其表现出不同的渗透适应性，并且其中转基因技术已经建立。因此，获得转ANP基因的青鳉，使其在没有转ANP基因的情况下不能在海水中生存，将直接证明ANP在海水适应中的重要性。然而，ANP的cDNA还没有被克隆青鳉鱼，虽然克隆是成功的，在其他几个硬骨鱼物种。同时，我们可以检测到四个CNP，命名为CNP-1至4，和B型NP（BNP），已被认为是四足特异性NP。此外，我们从最原始的现存脊椎动物，七鳃鳗和盲鳗中分离CNP-4型NP。因此，我们提出了NP家族分子进化的新假说：CNP-4是NP家族的一个祖先分子，在硬骨鱼中随着神经调节机制的进化而多样化。

项目成果

Anderson W.G.: "The control of drinking in elasmobranch fish with special reference to the rennin-angiotensin system. In : Osmoregulation and Drinking in Vertebrates"BIOS Scientific Publishers Ltd., Oxford. 1284 (2002)

Anderson W.G.：“软骨鱼饮水控制，特别涉及肾素-血管紧张素系统。见：脊椎动物的渗透调节和饮水”BIOS Scientific Publishers Ltd.，牛津。

Anderson W.G. et al.: "The dipsogenic effect of the rennin-angiotensin system in elasmobranch fish"General and Comparative Endocrinology. 125. 300-307 (2001)

Anderson W.G.等人：“软骨素-血管紧张素系统在软骨鱼类中的作用”普通和比较内分泌学。

Inoue K. et al.: "Asian medaka fishes offer new models for studying seawater adaptation"Comparative Biochemistry an Physiology. 136B. 635-645 (2003)

Inoue K.等人：“亚洲青鳉鱼为研究海水适应提供了新模型”比较生物化学和生理学。

Takei Y, et al.: "Mammalian homolog or fish adrenomedullin 2: Identification of a novel cardiovascular and renal regulator"Federation of European Biochemical Societies Letters. 556. 53-58 (2003)

Takei Y 等人：“哺乳动物同源物或鱼肾上腺髓质素 2：新型心血管和肾脏调节剂的鉴定”欧洲生化学会联合会快报。

Osman A. et al.: "Molecular identification and immunocytochemical localization of artial natriuretic peptide in the heart of camel"Comparative Biochemistry and Physiology. (IN PRESS).

Osman A. 等人：“骆驼心脏中人工钠尿肽的分子鉴定和免疫细胞化学定位”比较生物化学和生理学。