A novel myocardial protection with in vivo gene transfection

体内基因转染的新型心肌保护

基本信息

  • 批准号:
    06404047
  • 负责人:
  • 金额:
    $ 12.99万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    1994
  • 资助国家:
    日本
  • 起止时间:
    1994 至 1996
  • 项目状态:
    已结题

项目摘要

To develop a myocardial protection against is chemia-reperfusion injury, many kinds of pharmacological approaches have been tried. But they appear to have limited efficacy even now. Recently, another approach which uses self-preservation systems, such as is chemic preconditioning and heat shock, is expected to develop a further advanced myocardial protection. HSP70 is reported to be induced under various forms of stress such as ischemis, heat shock, or drug administration and enhance myocardial tolerance to ischemia-reperfusion injury. The object of this study is to develop a novel method for myocardial protection with gene transfection through enforcing the self-preservation system, such as HSP70. First, we isolated the role of HSP70 in coronary endothelial cells and in cardiac myocytes under conditions of ischemia-reperfusion injury using in vitro gene transfection. Next, we developed the oprimal method for in vivo gene transfection to whole heart by intracoronary infusion of HVJ-liposome. Then, we demonstrated that it is possible to introduce HSP70 into the entire heart with this gene transfection method and enhance myocardial tolerance to ischemia-reperfusion injury. These results showed the possibility of clinical application of gene therapy (long-term cardiac preservation) with HSP70 to ischemia-reperfusion injury of the heart.
为研究心肌缺血再灌注损伤的保护作用,人们尝试了多种药理学方法。但即使是现在,它们的功效似乎也有限。最近,另一种使用自我保护系统的方法,例如化学预处理和热休克,有望开发出更先进的心肌保护方法。热休克蛋白70(HSP 70)在缺血、热休克、药物等应激条件下均可诱导心肌细胞表达,并能增强心肌对缺血再灌注损伤的耐受性。本研究的目的是建立一种新的心肌保护方法,通过加强心肌细胞的自我保护系统,如热休克蛋白70基因转染。首先,我们分离的作用,热休克蛋白70在冠状动脉内皮细胞和心肌细胞在缺血再灌注损伤的条件下,通过体外基因转染。接下来,我们开发了通过冠状动脉内输注HVJ-脂质体进行体内基因转染至整个心脏的最佳方法。本研究证明了利用该基因转染方法将HSP 70导入整个心脏并增强心肌对缺血再灌注损伤的耐受性是可能的。这些结果表明,热休克蛋白70基因治疗(长期保存心脏)的心脏缺血再灌注损伤的临床应用的可能性。

项目成果

期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ken Suzuki: "In Vivo Gene Transfection with Heat Shock Protein 7C Enhances Myocardial Tplerance to Ischemia-Reperfusion Injury in Rat" The Journal of Clinical Investigation. (in press).
Ken Suzuki:“热休克蛋白 7C 体内基因转染增强大鼠心肌对缺血再灌注损伤的耐受性”《临床研究杂志》。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yoshiki Sawa: "Efficient transfer of oligonucleotide and plasmid DNA into whole heart through coronary artery (in press)" Journal of Thoracic and Cardiovascular Surgery.
Yoshiki Sawa:“通过冠状动脉将寡核苷酸和质粒 DNA 有效转移到整个心脏(正在印刷中)”《胸心血管外科杂志》。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
鈴木 憲: "遺伝子導入を応用した新しい心筋保護法の開発" 医学のあゆみ. 176. 764-765 (1996)
Ken Suzuki:“应用基因转移开发新的心肌保护方法”医学史 176. 764-765 (1996)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yoshiki Sawa: "Efficiency of In Vivo Gene Transfection Into Transplanted Rat Heart by Coronary Infusion of HVJ Liposome" Circulation. 92. 479-482 (1995)
Yoshiki Sawa:“通过冠状动脉输注 HVJ 脂质体将体内基因转染到移植的大鼠心脏中的效率”循环。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yoshiki Sawa: "Overexpression of HSP70 enhances myocardial tolerance to ischemia" Ischemic Heart. (in press).
Yoshiki Sawa:“HSP70 的过度表达增强心肌对缺血的耐受性”缺血性心脏。
  • DOI:
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  • 影响因子:
    0
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MATSUDA Hikaru其他文献

MATSUDA Hikaru的其他文献

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{{ truncateString('MATSUDA Hikaru', 18)}}的其他基金

Introduction of new strategy for end-stage heart failure by implantable ventricular assist device aiming to long-term-support with reintegration into society : Survey for the background and possible candidates.
通过植入式心室辅助装置引入治疗终末期心力衰竭的新策略,旨在为重新融入社会提供长期支持:调查背景和可能的候选人。
  • 批准号:
    21390396
  • 财政年份:
    2009
  • 资助金额:
    $ 12.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Myocardial regeneration therapy using nanotechnology enhances self-regeneration in damaged myocardium
使用纳米技术的心肌再生疗法可增强受损心肌的自我再生
  • 批准号:
    15209046
  • 财政年份:
    2003
  • 资助金额:
    $ 12.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
STUDIES FOR ESTABLISHMENT OF PEDIATRIC HEART AND LUNG TRANSPLANTATION IN JAPAN
日本开展儿科心肺移植的研究
  • 批准号:
    12307027
  • 财政年份:
    2000
  • 资助金额:
    $ 12.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Researches on novel techniques of organ-selective gene therapy in thoracic surgery
胸外科器官选择性基因治疗新技术研究
  • 批准号:
    11557100
  • 财政年份:
    1999
  • 资助金额:
    $ 12.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
MULTI-CENTER STUDIES FOR CLINICAL APPLICATION OF PEDIATRIC HEART AND LUNG TRANSPLANTATION IN JAPAN
日本小儿心肺移植临床应用多中心研究
  • 批准号:
    09307028
  • 财政年份:
    1997
  • 资助金额:
    $ 12.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
A novel gene therapy for congestive heart failure.
一种治疗充血性心力衰竭的新型基因疗法。
  • 批准号:
    08557079
  • 财政年份:
    1996
  • 资助金额:
    $ 12.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Research for natural prognosis and surgical effect in thoracic and abdominal aneurysm
胸腹动脉瘤自然预后及手术效果研究
  • 批准号:
    04557059
  • 财政年份:
    1993
  • 资助金额:
    $ 12.99万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
A clinical study on the mechamism and early diagnosis of acute liver dysfunction following cardiac surgery.
心脏术后急性肝功能障碍发生机制及早期诊断的临床研究
  • 批准号:
    62570636
  • 财政年份:
    1987
  • 资助金额:
    $ 12.99万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Assessment of the myocardial protection in critical neonates
危重新生儿心肌保护作用的评估
  • 批准号:
    60480317
  • 财政年份:
    1985
  • 资助金额:
    $ 12.99万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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