EXPERIMENTAL STUDY ABOUT THE INDUCTION AND MAINTENANCE OF IMMUNOLOGIC TOLERANCE FOR THE COMPOSITE TISSUE ALLOGRAFT

复合同种异体组织移植物免疫耐受诱导和维持的实验研究

• 批准号: 06404054
• 负责人: IKUTA Yoshikazu
• 金额: $ 8万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06404054/
• 关键词: ALLOGRAFT; TRANSPLANTATION IMMUNITY; TISSUE PRESERVATION; 組織保存

For the clinical application of composite tissue allograft, we have done the experimental stydy about allogenic limb transplantation in the rat using FK 506.1.FK 506 was effective in a dose lower than that of Cyclosporin A.2.Short course of FK 506 induced long-term graft survivals.3.Nerve regeneration and growth of the allografted limbs occured in the same manner as that of the autografted limbs.4.In the long-term survival group, grafted bone grew as fast as that of the autografted group.5.As a mechanism of the long-term graft survival achieved by FK 506, both the clonal anergy and clonal deletion of the lymphocytes were considered to be contributed.6.Functional loss of the replanted limbs was related to the free radicals, that was produced by the reperfusion.7.Though 6hrs' ischemia time under the warm condition for grafted limbs induced the closure of epiphyseal plate, over 9 hours' ischemia time under the cold condition prevented those growth disturbance.8.Vascularized free muscle grafts were effective for the dystrohbic mice.From now, bone marrow cell of donor, which was controlled at various concentrations, will be added to the recipient by the various way to induce immunotolerance at high ratio. After that, these results will be evaluated and we will esatbish the transplantation immunity for composite tissue allograft.

对于同种异体复合组织的临床应用，我们对FK 506进行了大鼠同种异体肢体移植的实验研究。1. FK 506在剂量低于环孢素A的情况下有效。2. FK 506短疗程诱导长时间的移植，3.同种异体移植肢体的神经再生和生长与自体移植肢体的神经再生和生长相同。FK506能使移植骨长期存活，其机制可能与淋巴细胞的克隆性无能和克隆性缺失有关。7.移植肢体在温热条件下缺血6h虽可引起骺板闭合，8.带血管蒂的游离肌肉移植对营养不良小鼠有良好的治疗效果，从目前来看，将其控制在不同的浓度下，通过各种方式添加到受体中，以高比例诱导免疫耐受。之后，我们将对这些结果进行评估，并评估复合组织同种异体移植物的移植免疫。

项目成果

Kuroki H,Ikuta Y: "Nerve Regenerations of Vasulkarized Rat Limb Allograft and Functional Recovery of Long-Term Graft Survivals Treated by Short Course of FK506 Or Cyelosporine." Transplant Proc. 27. 348-350 (1995)

Kuroki H、Ikuta Y：“通过短期 FK506 或环孢素治疗，血管化大鼠肢体同种异体移植物的神经再生和长期移植物存活的功能恢复。”

黒木秀尚: "同種複合組織移植の基礎的研究" 日本手の外科学会雑誌. 11. 100-100 (1994)

黑木秀久：“同种异体复合组织移植的基础研究”日本手外科学会杂志11. 100-100（1994）。

Kuroki H: "Nerve Regeneration of Vasculayized Rat Limb Allograft and Functional Recovery of Long-Term Graft Survivals Treated by Short Course of FK506" Transplantation Proceedings. 27. 348-350 (1995)

Kuroki H：“FK506 短期疗程治疗的血管化大鼠肢体同种异体移植物的神经再生和长期移植物存活的功能恢复”移植论文集。

黒木秀尚: "同種複合組織の基礎的研究" 日本手の外科学会雑誌. 11. 597-601 (1994)

Hidehisa Kuroki：“同质复合组织的基础研究”日本手外科学会杂志 11. 597-601 (1994)。

Dohi D: "Experimental challenge for the Treatment of Dushene Muscalar Atrophy using a Viasadarized Free Muscle Graft" Microsurgery. 15. 871-876 (1994)

Dohi D：“使用 Viasadarized 游离肌肉移植物治疗 Dushene 肌肉萎缩的实验挑战”显微外科手术。