SIGNIFICANCE OF NEUROPEPTIDE Y IN EARLY DIAGNOSIS AND PATHOGENETIC ANALYSIS OF NEUROBLASTOMA

神经肽Y在神经母细胞瘤早期诊断和发病机制分析中的意义

• 批准号: 06454503
• 负责人: HAYASHI Yutaka
• 金额: $ 4.1万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06454503/
• 关键词: NEUROBLASTOMA; PLASMA NPY; URINARY NPY; TISSUE NPY; IMMUNOHISTOCHEMISTRY Ki-67; Ki-67

Plasma neuropeptide Y (NPY) concentrations were detected in 63 cases with neuroblastoma (NB) and 64 cases without NB.Sensitivity and specificity of NPY on the diagnosis of NB were 86% and 82%, respectively. Plasma NPYs in Nb cases were significantly higher in cases of mass-screening negative cases, advanced ataged cases, high LDH or NSE cases, cases with UH of Shimada and N-myc amplified cases than counterparts of those cases.Urinary NPY was measured and NPY like immunoreactivity from 40 to 200 pg/ml in concentration was detected. It was found that major component of NPY in urine shifted to low molecular weight area. It was considered NPY to be metabolized at the urinary system and to be possible to measure urinary NPY concentrations. Urine NPY concentrations were detected in 17 cases with NB and 10 cases without NB.Urinary NPY concentrations in NB were not higher than those of other cases. Urinary NPYs significantly correlated with plasma NPYs but did not correlated with stages, ages, tumor sites and mass-screening.Immunohistochemistry of NPY and Ki-67 was done in 37 NB cases. Well differentiated tissues showed high NPY expression and low Ki-67 expression. Highly NPY positive tumor tissues tended to be lower Ki-67 expression.

检测了63例神经母细胞瘤(NB)患者和无神经母细胞瘤患者的血浆神经肽Y(NPY)浓度。NPY诊断NB的敏感性为86%，特异性为82%。血清NPY水平在大规模筛查阴性、晚期、LDH或NSE增高、岛田UH和N-myc扩增病例中均显著高于正常对照。发现尿液中NPY的主要成分向低分子量区转移。NPY被认为是在泌尿系统中代谢的，可以测量尿液中NPY的浓度。检测了17例NB患者和10例非NB患者的尿液NPY浓度。尿NPY与血浆NPY显著相关，与临床分期、年龄、肿瘤部位、筛查无关。对37例NB进行了NPY和Ki-67免疫组织化学染色。高分化组织中NPY高表达，Ki-67低表达。NPY高表达的肿瘤组织中Ki-67的表达往往较低。

项目成果

Hayashi Y,Ohi R,Tsuchiya S,et al :"Multidisciplinary treatment for advanced neuroblastoma : The eighth report from the Tohoku Study Group from April 1984 to January 1993" J Jpn Soc Pediatr Surg. 30. 924-929 (1994)

Hayashi Y，Ohi R，Tsuchiya S，et al：“晚期神经母细胞瘤的多学科治疗：东北研究组从 1984 年 4 月至 1993 年 1 月的第八次报告”J Jpn Soc Pediatr Surg。

林富: "進行再発癌の治療-神経芽細胞腫-" カレントテラピー. 14. 121-125 (1996)

Tomi Hayashi：“晚期复发性癌症 - 神经母细胞瘤的治疗”《当前疗法》14. 121-125 (1996)。

Satoh F,Takahashi K,Murakami O,et al :"Adrenomedullin in human brain, adrenal glands and tumor tissue of pheochromocytoma, ganglioneuroblastoma and neuroblastoma" J Clin Endocrinol Metab. 80. 1750-1752 (1995)

Satoh F，Takahashi K，Murakami O，等人：“人脑、肾上腺和嗜铬细胞瘤、神经节神经母细胞瘤和神经母细胞瘤肿瘤组织中的肾上腺髓质素”J Clin Endocrinol Metab。

林 富: "神経芽腫に対する18か月次児二次マススクリーニングの有効性と問題点" 小児がん. 33(2). 216-219 (1996)

Tomi Hayashi：“18 个月时神经母细胞瘤二次大规模筛查的功效和问题”，《儿科癌症》33(2) (1996)。

林富: "東北地区Group Stndyによる進行神経芽腫の治療(第8報):1984年4月〜1993年1月の総括" 日小外会誌. 30. 924-929 (1994)

Tomi Hayashi：“东北地区 Stndy 小组对晚期神经母细胞瘤的治疗（第 8 次报告）：1984 年 4 月至 1993 年 1 月的总结”日本外国语学会杂志 30. 924-929（1994）。