Development fo a column material for chromatographic resolution, using an ion-exchanged adduct of clay-alubumin.

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The present project has been initiated to develop a new column material for optical resolution, using an ion-exchange adduct of clay and albumin. A clay is a layr phyllosilicate capable of intercalating a positive ion in the interlayr spaces. Albumin is one of the globular proteins that have been utilized as a chiral modifier in a variety of chemical reactions. We aimed at combing the high ability of chiral recognition of albumin with the intercalating properties of a clay. In the first year of the project, we investigated the adsorption behaviors of albumin by colloidally dispersed sodium montmorillonite. As a result, it was revelealed that ablumin was adsorbed to an amount as high as 2g per 1g of a clay. In the second year, we attempted to see the reason for such high adsorption ability of a clay. A solid adduct of clay-albumin was investigated with solid state NMR and X-ray diffraction analyzes. In the next step, we prepared a column of the adduct for high performance liquid chromatography. As a result, we suceeded in resolving a useful chiral intermediate in organic synthese, benzoin, on the column. Finally we seaked for the optimum conditions for resolution, varying the solvent compositions, pH and temperature. The present results may open a way to develop a new colum for optical resolution on an industrial scale in a near future.