Development of Surface Nanostructure Control Method of Biomaterials by Utilizing Organosilane Monolayrs

利用有机硅烷单分子层控制生物材料表面纳米结构的方法的发展

• 批准号: 06558126
• 负责人: TAKAHARA Atsushi
• 金额: $ 3.07万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06558126/
• 关键词: Organosilane; Monolayr; Phase-separation; Surface Structure Control; Atomic Force Microscopy; Protein Adsorption; Patterning; Nanostructure; 有機シラン単分子膜; 相分離構造; 固定化単分子膜

Alkyltrichlorosilanes, [2- (perfluorooctyl) ethyl] trichlorosilane (FOETS) and their mixed monolayrs were polymerized on the water subphase and were covalently immobilized onto the silicon wafer surface. Atomic force microscopic (AFM) observation of the (n-octadecyltrichlorosilane (OTS) /FOETS) mixed monolayr revealed that the crystalline OTS formed circular domains of ca. 1-2 mum in diameter, which were surrounded by an amorphous FOETS matrix. Lateral force microscopic (LFM) and scanning viscoelastic microscopic (SVM) observations of the (OTS/FOETS) mixed monolayr could also distinguish the difference in the physical and mechanical properties between domain and matrix. The (alkylsilane/FOETS) mixed monolayrs with shorter alkyl chain such as n-dodecyltrichlorosilane (DDTS) did not show the phase-separated structure maybe due to the lack in crystallinity of DDTS component. AFM observation of the [crystalline fatty acid (non reactive) /FOETS (reactive)] mixed monolayr also revealed the phase-separated structure. The crystalline fatty acid domain in the mixed monolayr was extracted by solvent and then, the monolayr of FOETS with the holes of ca. 1-2 mum in diameter, that is, the (Si/FOETS) monolayr was finally obtained. The Si phase of (Si/FOETS) was backfilled with the another organosilane by chemisorption. Then, the [(3-mercaptopropyl) trimethoxysilane (MTS) /FOETS] mixed monolayr was newly obtained by the chemisorption of MTS.The adsorption of bovine serum albumin (BSA) onto mixed monolayrs was studied by exposure of the mixed monolayrs to a BSA solution. BSA was preferentially adsorbed onto the MTS part due to a specific reaction between disulfide bond (-S-S-) in BSA and SH groups in MTS.On the other hand, in the case of (OTS/FOETS) mixed monolayr, BSA adsorbed preferentially onto the FOETS matrix in order to minimized the interfacial free energy in the aqueous environment.

将烷基三氯硅烷、[2-（全氟辛基）乙基]三氯硅烷（FOETS）及其混合单分子膜在水亚相上聚合并共价固定在硅片表面。用原子力显微镜（AFM）对正十八烷基三氯硅烷（OTS）/FOETS混合单层膜进行了观察，发现OTS晶体形成了约1.5 μ m的环状结构。1-2μ m的直径，其中所包围的无定形FOETS矩阵。横向力显微镜（LFM）和扫描粘弹性显微镜（SVM）观察（OTS/FOETS）混合单层也可以区分域和基质之间的物理和机械性能的差异。具有较短烷基链的（烷基硅烷/FOETS）混合单分子膜如正十二烷基三氯硅烷（DDTS）没有显示出相分离结构，这可能是由于DDTS组分缺乏结晶性。[结晶脂肪酸（非反应性）/FOETS（反应性）]混合单层的AFM观察也揭示了相分离的结构。用溶剂抽提混合单层膜中的结晶脂肪酸结构域，然后用溶剂抽提具有约100个孔的FOETS单层膜。1-2μ m的直径，即，（Si/FOETS）单层，最终获得。（Si/FOETS）的Si相通过化学吸附被另一种有机硅烷回填。然后，通过化学吸附的方法制备了[（3-巯丙基）三甲氧基硅烷（MTS）/FOETS]混合单分子膜，研究了该混合单分子膜对牛血清白蛋白（BSA）的吸附行为。由于BSA中的二硫键（-S-S-）与MTS中的SH基团发生特异性反应，BSA优先吸附在MTS部分上，而在（OTS/FOETS）混合单层膜中，BSA优先吸附在FOETS基质上，以降低水环境中的界面自由能。

项目成果

T.Kajiyama: "Direct Observation of Surface Viscoelastic Properties of Polymeric Solids Based on Scanning Force Microscope" Proc.Japan Academy Ser.B. 71. (1995)

T.Kajiyama：“基于扫描力显微镜直接观察聚合物固体的表面粘弹性”Proc.Japan Academy Ser.B。

S.R.Ge: "Direct Observation of the Phase-Separated Structure in (Polyalkylsilane/Polyfluoroalkylsilane)Mixed Monolayer Surface by Scanning Force Micrsocopy" Rept.Progr.Polym Phys.Japan. 37. 315-318 (1994)

S.R.Ge：“通过扫描力显微镜直接观察（聚烷基硅烷/聚氟烷基硅烷）混合单层表面中的相分离结构”Rept.Progr.Polym Phys.Japan。

梶山千里: "新高分子実験学10巻第3章" 共立出版, 29 (1955)

梶山千里：《新高分子实验科学第10卷第3章》共立书刊，29（1955）

T.Kajiyama, K.Tanaka, I.Ohki, S.R.Ge, J.S.Yoon, A.Takahara: "Imaging of Dynamic Viscoelastic Properties of Phase Separated Polymer Surface by Forced Oscillation Atomic Force Microscopy" Macromolecules. Vol.27. 7931 (1994)

T.Kajiyama、K.Tanaka、I.Ohki、S.R.Ge、J.S.Yoon、A.Takahara：“通过强制振荡原子力显微镜对相分离聚合物表面的动态粘弹性进行成像”大分子。

S.-R.Ge,A.Takahara,T.Kajiyama: "Direct Observation of the Phase-separated Structure in (Polyalkylsilane/Polyfiuroalkylsilane) Mixed Monolayer Surface by Scanning Force Microscopy" Rept.Progr.Polym.Phys.,Japan. 37. 315-318 (1994)

S.-R.Ge，A.Takahara，T.Kajiyama：“通过扫描力显微镜直接观察（聚烷基硅烷/聚氟烷基硅烷）混合单层表面中的相分离结构”Rept.Progr.Polym.Phys.，日本。