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CONFIGURATION PATTERN OF CT-NUCLEOIDS IN DIATOMS

硅藻中 CT 核苷的构型模式

基本信息

批准号：
06804056
负责人：
MAYAMA Shigeki
金额：
$ 1.41万
依托单位：
TOKYO GAKUGEI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06804056/
关键词：
taxonomy systematics taxonomic characteristics chloroplast ct-nucleoid nucleoid diatom pyrenoid Nitzschia

项目摘要

Configurations of chloroplast-nucleoids (ct-nucleoid) were examined with DAPI-stained diatoms (16 genera, 50 species). The configuration was ring-type in all species except for several Pinnularia and Nitzschia species, in which scattered DAPI-fluorescent globules and short strings of DAPI-fluorescence were observed respectively. As DAPI-fluorescent globules in Pinnularia disappeared by DNase treatment, they were confirmed to contain DNA.These globules were confirmed to be located in the chloroplast by confocal laser microscopy and observations of the sections of the cells embedded in Technovit 7100. These globules corresponded to the discrete areas of thylakoids stack in TEM observation. No membranous structure was detected in these areas. The chloroplasts were isolated and then these globules also isolated. The size of globules were similar to that of each beads in ring-type nucleoid. Employing proteinase K treatment, these globules were lysed and then DNA was diffused. These observat … More ions and experiments prove that the globules scattered throughout the chloroplast are the nucleoids to be a complex of DNA and protein. In Pinnularia, 11 species have the ring-type ct-nucleoid and 9 species have the scattered-type nucleoid as well as the ring-type and 1 species has only the scattered-type. Species with the scattered-type are limited in diatoms of P.sect. Pinnularia and P.sect. Divergentissima. In Nitzschia sigmoidea, we observed that small rectangular pyrenoids were scattered throughout the chloroplasts by propion-carmine staining method. In TEM section, they were lens shape, which was typical in diatoms. Specific localization of Rubisco was detected in this structure using indirect immuno-fluorescence microscopy and electron microscopy with immuno-gold. Although the ring-type nucleoid was not observed in this species, linear DAPI-fluorescence was observed along both sides of the pyrenoids and they seem to be the ct-nucleoids. The configurations of ct-nucleoids in diatoms are diversified as in diatom frustules, and seem to reflect their systematics. Less

用DAPI染色的硅藻(16属，50种)研究了叶绿体类核(ct-核)的构型。除几种金针藻和菱形藻外，其余物种均为环状结构，分别观察到散在的DAPI荧光小球和短串的DAPI荧光。经DNA酶处理后，半夏DAPI荧光小球消失，证实含有DNA，激光共聚焦显微镜和Technovit 7100细胞切片观察证实这些小球位于叶绿体中。在透射电子显微镜观察中，这些小球与类囊体堆叠的离散区域相对应。在这些区域内未发现膜结构。叶绿体被分离，然后这些球体也被分离。球状颗粒的大小与环状类核中的每个珠状颗粒的大小相似。用蛋白酶K处理，这些小球被裂解，然后DNA扩散。…上的这些观察站更多的离子和实验证明，散布在叶绿体中的球状颗粒是DNA和蛋白质的复合体。在半边纲中，有11种具有环型ct-核，9种兼有散型和环型，1种只有散型。散落型的种类仅限于P.组的硅藻中。Pinnularia和P.Sect.多年生草药。用丙酮-胭脂红染色的方法观察到，在菱形藻的叶绿体中，有小的长方形蛋白核物质分布于整个叶绿体。在透射电子显微镜下，它们呈典型的硅藻透镜状。用间接免疫荧光显微镜和免疫胶体金电子显微镜检测到Rubisco在该结构中的特异性定位。虽然在该物种中没有观察到环状类核，但沿果核两侧观察到线形DAPI-荧光，它们似乎是ct-核。硅藻中类ct-核的构型和硅藻中的一样多种多样，似乎反映了它们的系统学。较少