DIVERSITY OF THE VALVE ONTOGENY IN DIATOMS AND ITS RELATIONSHIP TO THE PHYLOGENY

硅藻瓣膜个体发育的多样性及其与系统发育的关系

• 批准号: 12640677
• 负责人: MAYAMA Shigeki
• 金额: $ 2.3万
• 依托单位: TOKYO GAKUGEI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640677/
• 关键词: Endosymbiont; radiolaria; foraminifera; diatoms; biodiversity; Minutocellus; Pelagophyceae; marine plankton; 殻形成過程; Cocconeis; Navicula; Luticola; Pleurosira; 増大胞子

Marine and freshwater diatoms were collected from various places in Japan. They were isolated and cultured for the materials of morphological observations and molecular experiments. SEM observations of mature valves revealed some new structures in several species. In Cocconeis the forming valves and the developing perizonium of auxospore. The valve ontogeny of Eunotiashowed some similarity to araphid pennate diatoms in the middle stage of the development. But it showed ontogenetic similarity to biraphid diatoms in the late stages. Use of a fluorescent-label agent, which was taken into the forming valve, could distinguished the newly formed valves after nucleic division and valves already formed before nucleic division. The analyzes of 18S rDNA newly showed the phylogenetic position of Pseudostaurosira, Pseudohimantidium, Berkeleya, Diploneis, Luticola, three Cylindrofheca specis and Nitzschia palea. Close relationship between Pseudohimanthidium and Eunotia, that had been pointed out based on morphology, was not shown in the phylogenetic trees, as Pseudohimantidium formed monophyly with other araphid marine genera. Except for the relationship between Bacillariaceae and Eunotiaceae, which is an opportunist according to the number of OUT and the methods of phylogenetic analyzes, the branching order of the higher rank of diatom taxa reflected that of the ontogenetic similarities from the early to middle stages of the taxa. Sill-like DNA sequences decided in five diatoms showed the high similarity among them, impling its possibility to become a probe for exploring the origin of the biosilica mineralization ability.

从日本各地收集海洋和淡水硅藻。分离培养，为形态学观察和分子生物学实验提供材料。成熟瓣的扫描电镜观察揭示了一些新的结构在几个物种。蚧壳属中形成的裂孔和发育中的孢子周层。Eunotia的瓣体发育在中期阶段表现出与Araphid pennate diatoms的相似性。但在后期阶段与双针硅藻的个体发育相似。使用荧光标记剂，它被带到形成阀，可以区分核分裂后新形成的阀和核分裂前已经形成的阀。18S rDNA分析新揭示了拟十字链藻属、拟半甲藻属、Berkeleya属、双壁藻属、Luticola属、三种菱藻属和内菱形藻属的系统发育位置。在系统发育树中，没有显示出形态学上指出的拟扁蚜属与真蚜属的亲缘关系，而是与其他的海生属形成了单系。除硅藻科和真硅藻科的OUT数和系统发育分析结果表明它们之间的亲缘关系为机会主义外，硅藻高级别分类群的分支顺序反映了该分类群早期至中期个体发育相似性的顺序。5种硅藻的类硅质DNA序列具有较高的相似性，有望成为探索生物硅矿化能力起源的探针。

项目成果

Mayama, S.: "Valuable taxonomic characters in the valve mantle and girdle of some Eunotia species"In : Jahn, R., Kociolek, J.P., Witkowski, A.&P.Compere (eds) Lange-Bertalot-Festschrift. Gantner, Ruggell. 119-130 (2001)

Mayama, S.：“一些 Eunotia 物种的瓣膜地幔和带的有价值的分类学特征”，见：Jahn, R.、Kociolek, J.P.、Witkowski, A.

鈴木 秀和, 南雲 保: "神奈川県葉山産の紅藻ピリヒバに着生する珪藻類"日本歯科大学紀要, 一般教育系. 32. 109-118 (2003)

Hidekazu Suzuki、Tamotsu Nagumo：“在神奈川县叶山的红藻 Pirihiba 上生长的硅藻”日本齿科大学通报，普通教育部 32. 109-118 (2003)。

Idei, M, Mayama, S.: "Pinnularia acidojaponica M. Idei et H.Kobayasi sp. nov. and P. valdetolerans Mayama et H. Kobayasi sp. nov. -new diatom taxa from Japanese extreme environments"In : Jahn, R., J.P. Kociolek, A. Witkowski & P.Compere (eds) Lange-Bertal

Idei, M, Mayama, S.：“Pinnularia Acidojaponica M. Idei et H.Kobayasi sp. nov. 和 P. valdetolerans Mayama et H. Kobayasi sp. nov. - 来自日本极端环境的新硅藻类群”In：Jahn, R

Idei, M., Mayama, S.: "Pinnularia acidojaponica M. Idei et H. Kobayashi sp. nov. and P. valdetolerans Mayama et H. Kobayashi sp. nov. - new diatom taxa from Japanese extreme environments"Lange-Bertalot-Festschrift. Studied on Diatoms. In J. Jahn et al.(ed

Idei, M., Mayama, S.：“Pinnularia Acidojaponica M. Idei et H. Kobayashi sp. nov. 和 P. valdetolerans Mayama et H. Kobayashi sp. nov. - 来自日本极端环境的新硅藻类群”Lange-Bertalot-

南雲保,真山茂樹.: "沖縄県瀬底島沿岸の海産珪藻類."Diatom. 16. 11-17 (2000)

Tamotsu Nagumo，Shigeki Mayama.：“冲绳县濑底岛沿岸的海洋硅藻。” 16. 11-17 (2000)