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STUDIES OF BIODIVERSITY ON PROTOZOAN ENDOSYMBIOTIC DIATOMS

原生动物内共生硅藻生物多样性研究

基本信息

批准号：
10836008
负责人：
MAYAMA Shigeki
金额：
$ 1.73万
依托单位：
Tokyo Gakugei University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10836008/
关键词：
Endosymbiont radiolaria forminifera diatoms biodiversity Minutocellus Pelagophyceae marine plankton Nannochloropsis

项目摘要

Endosymbiotic diatoms were isolated from radiolarian and foraminiferan hosts and cultured. Diatoms identified were Nitzschia sp., Entomoneis sp. Nanofrustulum shiloi from benthic larger foraminifera, Sorites orbiculus, and Amphora roettgeri from planktonic foraminifera, Globigerina sp. Endosymbiotic diatom from Sphaerozoum fuscum was Minutocellus polymorphus and observed in detail with transmission and scanning electron microscopy. It was clarified in the analysis of 18S rDNA, that M. polymorphs formed monophyly with Cymatosira and Papilocellulus, which are morphological members of Cymatosiraceae, Symbiotic alga isolated from another Globigerina sp. was cultured and examined by TEM. It was coccoid Pelagophyceae and similar to Pelagococcus subviridis in cellural fien structure but to Pelagococcus calceolata in the analyses of 18S rDNA.We explored transformation route of algae to radiolarian cell inside. Radiolarian species of Spumellaria and Nassellaria were studied regarding relative size to endosymbionts, presence or absence of the opening of radiolarian shell and the size of the opening. Moreover, the diversity of distom flora along the Japan Current flowing from tropical sea area was studied, since it is important for the source of symbionts. Shikine Island and Ryukyu Islands were chosen as study area an researches were performed over two years.

从放射虫和有孔虫宿主中分离出内共生硅藻并进行培养。硅藻鉴定为菱形藻属，利用透射电镜和扫描电镜对采自底栖大型有孔虫的Entomoneis sp. Nanofrustulum shiloi、采自浮游有孔虫的Sorites orbiculus和Amphora roettgeri、采自Sphaerozoum fuscum的Globigerina sp.内共生硅藻为Minutocellus polymorphus进行了详细观察。18S rDNA分析表明，M.多型体与Cymatosiraceae的形态学成员Cymatosira和Papilocellulus形成单系，从另一种Globigerina sp.分离的共生菌进行培养，并通过透射电镜进行观察。该藻属球形藻纲，其细胞形态结构与亚绿色藻（Pelagococcus subviridis）相似，但18S rDNA分析结果与灰绿藻（Pelagococcus calceolata）相似。研究了Spumellaria和Nassellaria的放射虫种与内共生体的相对大小、放射虫壳开口的存在与否以及开口的大小。此外，还研究了从热带海域流出的日本海流沿着远缘植物群的多样性，因为它是共生体的重要来源。本研究以志根岛与琉球群岛为研究区域，进行了两年多的研究。