Analytical epidemiological study on relationship between HCV,ALDH2 and hepatocellular carcinoma

HCV、ALDH2与肝癌关系的流行病学分析研究

• 批准号: 06670392
• 负责人: FUKUDA Katsuhiro
• 金额: $ 1.41万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06670392/
• 关键词: HCV; ALDH2; HCC; case control study; community control; nail; alcohol drinking; PCR

(1) ALDH2 genotyping method based on nail clippings has been developed using PCR.(2) Since June 1992, a case-control study has been performed on primary hepatocellular carcinoma (HCC) in Fukuoka and Saga Prefecture under a Grant-in-Aid for Scientific Research. Until the end of 1996,193 pairs of case, age-, sex-, residence-matched hospital-based control (HC) have been identified and their data e.g. HBsAg, anti-HCVAb, have been completed by a stuctured interview.(3) During the term between June 1992 and July 1995,146 HCC cases were identified and their equal number of age-, sex-matched community controls (OC) were randomly sampled from the alive Kurume citizens in June 1995 and their relevant data was then obtained by a mailed questionnaire. This procedure yielded 146 triplets (male 115, female 31) of case and the two types of controls.(4) Proportion of HBsAg positive among 115 male cases or HCs was 13.0%, 0.9%, and that of anti-HCVAb among cases and HCs was 83.5%, 7.0% respectively. Proportion of heavy drinker who consumed alcohol more than 60 drink-years by age 40 among cases, HCs and CCs was 23.5%, 13.9%, 9.6% respectively. Proportion of ALDH2 typical homozygote among 60 cases, HCs, and CCs was 58.3%, 63.3%, 38.3% respectively.(5) Univariate analysis using logistic regression model revealed that odds ratios of HBsAg positive, anti-HCVAb positive, and ALDH2 typical homozygote regarding developing male HCC were 15 (P<0.05 ; based on HC), 89 (P<0.05 ; based on HC), 2 (P<0.05 ; based on CC) respectively.

(1)利用PCR技术建立了基于指甲剪报的ALDH2基因分型方法。(2) 1992年6月以来，在一项科学研究资助下，在福冈和佐贺县进行了原发性肝细胞癌（HCC）的病例对照研究。截至1996年底，已确定了193对年龄、性别、居住地相匹配的医院对照（HC），并通过结构化访谈完成了他们的数据，如HBsAg、抗hcvab。(3)在1992年6月至1995年7月期间，共鉴定出146例HCC病例，并于1995年6月从库鲁姆市在世居民中随机抽取了等量的年龄、性别匹配的社区对照（OC），通过邮寄问卷的方式获得相关数据。该方法共获得病例三胞胎146例（男115例，女31例）和两种对照。(4) 115例男性hcc患者中HBsAg阳性的比例分别为13.0%、0.9%，抗hcvab阳性的比例分别为83.5%、7.0%。40岁时饮酒超过60年的重度饮酒者占病例、hc和cc的比例分别为23.5%、13.9%和9.6%。60例中ALDH2典型纯合子比例为58.3%，hcc为63.3%，cc为38.3%。(5)单因素logistic回归分析显示，HBsAg阳性、抗hcvab阳性、ALDH2典型纯合子与男性HCC发生的比值比分别为15（基于HC）、89 （P<0.05）、2 （P<0.05）。

项目成果

Shibata, A., Fukuda, K: "Effect of ALDH2 genotype and alcohol drinking on hepatocellular carcinoma" 55^<th> Annual Meeting of Japanese Cancer Association Yokohama. (1996)

Shibata, A.，Fukuda, K：“ALDH2 基因型和饮酒对肝细胞癌的影响”第 55 届日本癌症协会横滨年会。

Fukuda, K., Shibata, A.et al: "A case control study on male hepatocellular carcinoma based on hospital and community controls" 14^<th> International Scientific Meeting of the International Epidemiological Association, Nagoya. (1996)

Fukuda, K.、Shibata, A.等人：“基于医院和社区控制的男性肝细胞癌病例对照研究”，第 14 届国际流行病学协会国际科学会议，名古屋。

柴田 彰: "ALDH2の遺伝子型がアルコール飲酒と肝癌との関連に及ぼす影響" 第55回日本癌学会総会、横浜. 55. 675-675 (1996)

Akira Shibata：“ALDH2 基因型对饮酒与肝癌之间关系的影响”第 55 届日本癌症协会年会，横滨 55. 675-675 (1996)。

Nishiyori, A., Fukuda, K.et al: "Genotype-Phenotype Agreement of Aldahyde Dehydrogenase 2 in 120 healthy Japanese" Kurume Medical Journal. 41. 117-121 (1994)

Nishiyori, A.、Fukuda, K.等人：“120 名健康日本人中醛脱氢酶 2 的基因型-表型一致性”久留米医学杂志。

Nishiyori,A.: "Genotype-Phenotype Agreement of Aldehyde Dehydrogenase 2 in 120 healthy Japanese" Kurume Medical Journal. 41. 117-121 (1994)

Nishiyori,A.：“120 名健康日本人中醛脱氢酶 2 的基因型-表型一致性”久留米医学杂志。