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The effect of eosinophilopoietic cytokines on eosinophil CD32 expression in atopic dermatitis.

嗜酸性粒细胞细胞因子对特应性皮炎中嗜酸性粒细胞 CD32 表达的影响。

基本信息

批准号：
06670890
负责人：
MIYASATO Minoru
金额：
$ 1.28万
依托单位：
Kurume University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06670890/
关键词：
A topic dermatitis Eosinophil CD32 Eosinophilopoietic cytokine House dust mite IgG subclass アトピー性皮膚炎好酸球ダニ抗原 HDM-specitic IgG Eosinophil antigen

项目摘要

In a series of studies on eosinophils in patients with atopic dermatitis (AD), we obtained the results as follows ; 1)A simple method was established for the procurement of human blood eosinophils by combining an anti-CD16, immunobeads, and Nycodenz density gradient medium ; 2) Human eosino-phils expressed CD32 and CD35, but not CD23 and CD64 ; 3) In AD patients, CD32 expression of eosinophils was increased in correlation to the extent of blood eosinophilia (and disease severity) ; 4) Plasma levels of eosinophilopoietic cytokines (IL-3, IL-5 and GM-CSF) were within normal ranges. Observation that ricombnant human eosinophilopoietic cytokines did not affected on the expression of CD32 on eosinophils from normal subjects suggests that eosinophil CD32 expression may be regulated within the bone marrow ; 5) Sera from AD patients contained the house dust mite (HDM)-specific IgE and IgG antibodies. Of the two HDM-specific antibodies, HDM/IgG immune complex strongly induced eosinophil degranulation ; 6) Chemiluminescence procedure was established for determination of HDM-specific IgG subclasses, and then it was disclossed that plasma levels of IgG1 and IgG3 significantly correlated with the disease severity ; and 7) There was a high degree of correlation between plasma levels of IL-10 and plasma levels of IgG1 and IgG3, suggesting that IL-10 may implicate in the production of these HDM-specific IgG subclasses in AD patients.

在对特应性皮炎（AD）患者嗜酸性粒细胞进行的一系列研究中，我们获得了以下结果； 1)结合抗CD16、免疫珠和Nycodenz密度梯度介质，建立了一种简单的人血嗜酸性粒细胞获取方法； 2）人嗜酸性粒细胞表达CD32和CD35，但不表达CD23和CD64； 3）在AD患者中，嗜酸性粒细胞的CD32表达增加，与血液嗜酸性粒细胞增多的程度（和疾病严重程度）相关； 4) 血浆嗜酸性细胞因子（IL-3、IL-5和GM-CSF）水平在正常范围内。观察到重组人嗜酸性粒细胞细胞因子不影响正常人嗜酸性粒细胞上CD32的表达，这表明嗜酸性粒细胞CD32的表达可能在骨髓内受到调节。 5) AD 患者的血清含有屋尘螨 (HDM) 特异性 IgE 和 IgG 抗体。在两种 HDM 特异性抗体中，HDM/IgG 免疫复合物强烈诱导嗜酸性粒细胞脱颗粒； 6）建立了化学发光测定HDM特异性IgG亚类的方法，发现血浆IgG1和IgG3水平与疾病严重程度显着相关； 7) IL-10 血浆水平与 IgG1 和 IgG3 血浆水平之间存在高度相关性，表明 IL-10 可能与 AD 患者中这些 HDM 特异性 IgG 亚类的产生有关。