c-fos protein activation and functional analysis in central nervous system after transcranial magnetic stimulation

经颅磁刺激后中枢神经系统c-fos蛋白激活及功能分析

• 批准号: 06671446
• 负责人: KITAGAWA Hideki
• 金额: $ 1.28万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06671446/
• 关键词: Magnetic stimulation; c-fos protein; motor evoked potential; C-fos遺伝子; 磁気刺激

Neural activation and conducting pathways after transcranial magnetic stimulation were studied by c-fos protein analysis and evoked spinal cord and evoked compound muscle action potential (ESCP and ECMAP) analysis. c-fos protein was stained in the nucleus of pyramidal and extrapyramidal tracts, which was observed in the nucleus over the entire cerebral cortex. Therefore, transcranial magnetic stimulation was supposed to be spread not only the motor cortex but also the entire cerebral cortex.To obtain the optimal conditions of the transcranial magnetic stimulation in cats. ECMAP and ESCP in response to transcranial magnetic stimulation, and magnetic and electric field were analyzed in cats with and without permalloy shield.Ten adult cats were anesthetized with ketamine and subjected to transcranial magnetic stimulation with an 8 figure magnetic coil under a shield of permalloy that had variable windows open to the cortical motor area.Firstly, ECMAP and ESCP were recorded with an 8 figur … More e coil under no permalloy shield or under various windows of permalloy shield. Secondly, because magnetic field shield, or permalloy, altered the size and homogeneity of the magnetic field, magnetic and electric fields under magnetic coil were studied with and without permalloy.The maximum amplitude of ECMAP was recorded when the coil was placed on-catユs cranium at an angle of 90 degrees with and without a permalloy shield with a 5x5 cm window. Thresholds of ECMAP and ESCP were lower under a permalloy shield with a 5x5 cm window than without a permalloy. Strength of magnetic and electric field were highest at the brain stem area. Thus, large induced electric field directed caudally elicited ECMAP and ESCP effectively at a coil angle of 90 degrees with a 5x5 cm window permalloy.Transcranial magnetic stimulation revealed diffuse c-fos protein activity of the nucleus in the motor cortex, diffuse cerebral cortex, and brain stem. Modified electric field conceivably stimulated centrifugal pathways at the vicinity of the brain atem and entire cerebral cortex. Less

采用c-fos蛋白分析、诱发脊髓和诱发复合肌肉动作电位（ESCP和ECMAP）分析等方法研究经颅磁刺激后神经元的激活和传导通路。c-fos蛋白在锥体束和锥体外束的核中染色，其在整个大脑皮层的核中观察到。因此，经颅磁刺激不仅要在运动皮层，而且要在整个大脑皮层进行，以获得猫经颅磁刺激的最佳条件。本实验观察了猫在有、无坡莫合金屏蔽时对经颅磁刺激的ECMAP和ESCP的反应。10只成年猫用氯胺酮麻醉后，在坡莫合金屏蔽下用一个8字线圈进行经颅磁刺激，屏蔽窗开有不同的窗口，向大脑皮层运动区开放。首先用8字线圈记录ECMAP和ESCP，然后用磁刺激仪记录ECMAP和ESCP。结果表明，在有、无坡莫合金屏蔽时，ECMAP和ESCP的变化与磁刺激的变化有关。 ...更多信息 E线圈在没有坡莫合金屏蔽下或在坡莫合金屏蔽的各种窗口下。其次，由于磁场屏蔽或坡莫合金改变了磁场的大小和均匀性，因此研究了有和没有坡莫合金的磁性线圈下的磁场和电场，当线圈以90度角放置在猫颅骨上时，记录了ECMAP的最大振幅，有和没有坡莫合金屏蔽，窗口为5x 5cm。在具有5x 5 cm窗口的坡莫合金防护罩下，ECMAP和ESCP的有效值低于无坡莫合金防护罩的有效值。磁场和电场强度在脑干区最高。因此，大的诱导电场指向尾部引起ECMAP和ESCP有效地在90度的线圈角度与5 × 5 cm的窗口permalloy.Transcranial磁刺激显示弥漫性c-fos蛋白活性的运动皮层，弥漫性大脑皮层，和脑干的核。可以想象，改良电场刺激了脑干附近和整个大脑皮层的离心通路。少

项目成果

Hideki Kitagawa: "Magnetic evoked compound muscle action potential neuromonitoring in spine surgery" Spine. (in press).

北川秀树：“脊柱手术中的磁诱发复合肌肉动作电位神经监测”脊柱。

Hideki Kitagawa, MD.: "c-fos protein activation in central nervous system after transcranial magnetic stimulation in cats." in preparation for submission to EEG and EMG,(in Japanese). (1997)

Hideki Kitakawa，医学博士：“猫经颅磁刺激后中枢神经系统中的 c-fos 蛋白激活。”

Hideki Kitagawa, MD,Shinichi Nakatoh, MD,Yoshiharu Kawaguchi, MD,Hiroshi Nakamura, MD,and Haruo Tsuji, MD: "Effects of coil orientation and magnetic field shield on transcranial magnetic stimulation in cats." submitted in Muscle and nerve. (1997)

Hideki Kitakawa（医学博士）、Shinichi Nakatoh（医学博士）、Yoshiharu Kawaguchi（医学博士）、Hiroshi Nakamura（医学博士）和 Haruo Tsuji（医学博士）：“线圈方向和磁场屏蔽对猫经颅磁刺激的影响。”

Kitagawa H.: "Effects of coil orientation and magnetic shield to transcranial magnetic stimulation in cats." Muscle and nerve. (予定).

Kitakawa H.：“线圈定向和磁屏蔽对猫经颅磁刺激的影响（计划）。”

中藤真一、高野治雄、北川秀機、中村 宏: "パ-マロイによるネコ経頭蓋磁気刺激のいき値変化." 脊髄電気診断学. 17. 33-36 (1995)

Shinichi Nakafuji、Haruo Takano、Hideki Kitakawa、Hiroshi Nakamura：“坡莫合金对猫经颅磁刺激的阈值变化。”17. 33-36 (1995)