Activation and function of immunoglobulin binding factor in human seminal plasma.

人精浆中免疫球蛋白结合因子的激活和功能。

• 批准号: 06671655
• 负责人: KAMADA Masaharu
• 金额: $ 1.34万
• 依托单位: The University of Tokushima, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06671655/
• 关键词: immunoglobulin binding factor; antisperm antibody; sperm coating antigen; uterine cervical gland; tryptase; molecular chaperon; protein disulfide isomerase; seminal plasmacervical mucous; 不妊症; 分子シャペロン; 局所免疫; 子宮頸管; 頸管粘液; 気管; 喫煙; 蛋白分解酵素; アルギ

Immunoglobulin binding factor (IgBF) of human seminal plasma may be involved in suppressing antibody production against sperm in the female and male genital tracts. However, purified IgBF of seminal plasma was inactive homodimer. In the present project, we examined its localization, especially in the female genital tract, and activation mechanisms of homodimer form of IgBF.Results obtained are as follows ;1.High level of IgBF was found in cervical mucus of the uterus and bronchial secretion by ELISA.2.Expression of IgBFmRNA was observed in the uterine cervical gland by RTPCR and in situ hybridization.3.Homodimer form IgBF was present on the sperm surface as the sperm coating antigen by immunofluorescence study.4.Inactive homodimer form of IgBF was activated by various conditions, such as under the presence of glutathione, treatment with arginilendopeptidase (originated from parotic gland) or tryptase TL-1 (originated from T cell) and change of the conformation by molecular chaperon (protein disulfide isomerase).These findings may support a part of the mechanisms suppressing the antibody production against sperm in women. Namely, enough amount of IgBF is present in the female genital tract by local production and by transportation of prostatic IgBF into the tract as the sperm coating antigen, and activated locally to suppress B cell function.

人精浆中的免疫球蛋白结合因子（IgBF）可能参与抑制女性和男性生殖道中抗精子抗体的产生。精浆IgBF为非活性同二聚体。在本项目中，我们研究了它的定位，特别是在女性生殖道中，以及IgBF的同二聚体形式的激活机制。得到的结果如下：1。elisa法检测宫颈黏液和支气管分泌物中IgBF水平较高。采用RTPCR和原位杂交技术观察IgBFmRNA在子宫颈腺组织中的表达。免疫荧光法检测到IgBF同型二聚体作为精子包衣抗原存在于精子表面。无活性的IgBF同二聚体形式在多种条件下被激活，如谷胱甘肽存在下，精氨酸内肽酶（起源于腮腺）或胰蛋白酶TL-1（起源于T细胞）处理以及分子伴侣（蛋白质二硫异构酶）改变构象。这些发现可能支持部分抑制女性精子抗体产生的机制。即，IgBF在女性生殖道内通过局部产生和作为精子包被抗原的前列腺IgBF转运进入生殖道，并在局部活化抑制B细胞功能。

项目成果

Hirano M.: "Significance of immunoglobulin binding factor as the sperm coating antigen" Proceedings of the 9th Annual Meeting of JSIR. 9. 25-26 (1995)

Hirano M.：“免疫球蛋白结合因子作为精子包被抗原的意义”，JSIR 第九届年会论文集。

Negishi,H.: "Impaired infertility in female rats immunized with synthetic peptide segments of two sperm antigens." Biochem.Arch.12. 89-94 (1996)

Negishi,H.：“用两种精子抗原的合成肽片段免疫雌性大鼠的不育症。”

Nakaya, Y.: "Inducible nitric oxide synthase in uterine smooth muscle." Life Science. 58. 249-255 (1996)

Nakaya, Y.：“子宫平滑肌中的诱导型一氧化氮合酶。”

Kamada, M.: "Autoantibodies to zona pellucida" In Immunocontraception Ed.by O.Nilsson and R.Mattsson Ares Serono Symposia Pub.Rome, Italy. 99-11 (280)

Kamada, M.：“透明带自身抗体”，免疫避孕版，作者：O.Nilsson 和 R.Mattsson Ares Serono Symposia Pub，意大利罗马。

Yamamoto, S.: "Demonstration of anti-fortility effect by biologically active antioperm antibodies and its failure by sperm - binding antibodies" Proceedings of the 10th Annual Meeting of JSIR. (in press).

Yamamoto, S.：“生物活性抗精子抗体的抗生育效应及其精子结合抗体的失败示范”JSIR 第十届年会记录。