Role of dendritic cells as adjuvants and carrier of microbial antigens in mucosal immunization

树突状细胞作为微生物抗原佐剂和载体在粘膜免疫中的作用

• 批准号: 5266774
• 负责人: Professor Dr. Horst Ulrich Beuscher
• 金额: --
• 依托单位: Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2000
• 资助国家: 德国
• 起止时间: 1999-12-31 至 2004-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5266774?language=en
• 关键词: Role; dendritic; cells; adjuvants; carrier

Mucosal immunization can elicit protection against infection at mucosal surfaces as well as systemically. With soluble antigenes, however, it has been difficult to induce antigene specific immunity, as prolonged feeding or large oral doses of protein antigenes may induce oral tolerance. Here we describe a novel approach for mucosal immunization by using ex vivo antigene-pulsed dendritic cells (DC). Infection of mice with Yersinia enterocolitica serves as a model system. Yersinia-derived antigenes are available as recombinant proteins and have been previously characterized as major virulence proteins. Specific aims include characterization of the immunogenicity of in vitro antigene-pulsed DCs, analysis of migration patterns of antigene-pulsed DCs in mucosal tissues and evaluation of their efficacy in the induction of local and systemic immunity. The data will contribute to the understanding of the immunomodulating properties of antigene-pulsed DCs in mucosal tissues. They also might provide clues for the design of vaccines capable of targeting specific immunity to mucosal surfaces.

粘膜免疫可以在粘膜表面和系统内诱导对感染的保护。然而，对于可溶性抗基因，很难诱导抗基因特异性免疫，因为长时间喂养或大剂量口服蛋白质抗基因可能会诱导口服耐受。在这里，我们描述了一种利用体外抗基因冲击的树突状细胞(DC)进行粘膜免疫的新方法。以小鼠感染小肠结肠炎耶尔森氏菌为模型系统。耶尔森氏菌来源的抗菌素以重组蛋白的形式存在，以前已被鉴定为主要的毒力蛋白。具体目标包括体外抗基因冲击DC的免疫原性鉴定，分析抗基因冲击DC在黏膜组织中的迁移模式，以及评估其在诱导局部和系统免疫方面的效果。这些数据将有助于理解抗基因冲击的DC在黏膜组织中的免疫调节特性。它们还可能为设计能够针对粘膜表面的特定免疫的疫苗提供线索。